脂肪源干细胞-聚丙烯复合网片的生物相容性实验研究

发布时间：2018-03-13 10:46

本文选题：脂肪源干细胞　切入点：组织工程　出处：《医学研究生学报》2015年07期 　论文类型：期刊论文

【摘要】：目的如何避免合成补片与人体组织产生的排异反应是目前急需解决的问题,文中探讨脂肪源干细胞(adipose derived stem cells,ADSCs)与聚丙烯网片在动物体内的生物相容性。方法制备兔脂肪源性干细胞悬液。ADSCs传代扩增后,接种到聚丙烯网片支架上,体外培养1周。分别将聚丙烯网片、ADSCs-聚丙烯复合网片植入家兔的腹直肌表面,4周后观察网片腐蚀、粘连情况,HE染色进行组织学观察,采用RT-PCR检测VEGF mRNA的表达水平。结果流式细胞仪检测结果提示ADSCs CD90、CD44、CD73、CD45、CD34及CD14表达分别为98.54%、95.32%、98.49%、1.21%、3.01%、2.14%。聚丙烯网片、ADSCs-聚丙烯复合网片均有不同程度的腐蚀、粘连,但聚丙烯网片粘连更致密。与聚丙烯比较,ADSCs-聚丙烯复合网片诱导慢性炎症反应轻,炎症评分降低[(1.1±0.2)分vs(0.6±0.1)分,P=0.001],新生血管形成评分升高[(1.7±0.0)分vs(2.6±0.3)分,P=0.000],成纤维细胞增殖评分升高[(0.9±0.1)分vs(2.2±0.2)分,P=0.001]。ADSCs-聚丙烯复合网片周围组织VEGF mRNA含量高于聚丙烯网片(t=94.6,P0.05)。结论 ADSCs-聚丙烯复合网片具有良好的生物相容性,可作为脂肪组织工程较理想的生物支架材料。
[Abstract]:Objective how to avoid the rejection reaction between synthetic patch and human tissue is an urgent problem. The biocompatibility of adipose derived stem cells (ADSCs) and polypropylene mesh in animals was studied. After cultured in vitro for 1 week, the ADSCs- PP composite mesh was implanted into rabbit rectus abdominis for 4 weeks. The corrosion of the mesh was observed, and the adhesion was observed by HE staining. RT-PCR was used to detect the expression of VEGF mRNA. Results the results of flow cytometry showed that the expression of ADSCs CD90, CD44, CD74, CD45, CD45, CD34 and CD14 were 98.5454 and 95.32, respectively. But the adhesion of polypropylene mesh was denser than that of polypropylene. The inflammation score decreased [1. 1 卤0. 2) vs(0.6 卤0. 1 min P0. 001], the neovascularization score increased [1. 7 卤0. 0) vs(2.6 卤0. 3], the fibroblast proliferation score increased [0. 9 卤0. 1 vs(2.2 卤0. 2]. VEGF mRNA content in the surrounding tissue of ADSCs- polypropylene composite mesh was higher than that in polypropylene mesh. Propylene composite mesh has good biocompatibility. It can be used as an ideal scaffold material for adipose tissue engineering.
【作者单位】：徐州市中心医院妇产科;
【基金】：江苏省医学重点人才基金(RC2011047) 徐州市中心医院创新团队科技项目(xzs2012036)
【分类号】：R711.5

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