c-cbl基因沉默对卵巢癌SKOV3细胞增殖和侵袭的影响

发布时间：2018-03-14 13:55

本文选题：c-cbl　切入点：卵巢肿瘤　出处：《重庆医学》2015年36期 　论文类型：期刊论文

【摘要】：目的探讨c-cbl在卵巢癌的表达及其对卵巢癌细胞增殖、侵袭的影响。方法通过免疫组织化学法(S-P法)检测c-cbl蛋白在卵巢癌组织的表达;EdU检测卵巢癌SKOV3细胞沉默c-cbl基因后细胞增殖能力变化;Transwell检测卵巢癌SKOV3细胞侵袭能力;Western blot检测P21蛋白和P53蛋白的表达。结果 c-cbl蛋白在卵巢癌中表达位于细胞质,在卵巢癌中,c-cbl蛋白呈强阳性表达,在正常卵巢组织c-cbl蛋白呈弱阳性或者阴性表达。与正常卵巢组织比较,c-cbl蛋白在卵巢癌表达明显增高(P0.05);c-cbl蛋白在卵巢癌的表达与FIGO分期相关(P0.05);沉默卵巢癌SKOV3细胞c-cbl基因后,卵巢癌细胞增殖能力和侵袭能力降低(P0.05);沉默c-cbl基因后,P21和P53蛋白表达上调(P0.05)。结论 c-cbl蛋白在卵巢癌表达上调,沉默c-cbl基因可能与上调P21和P53蛋白有关。
[Abstract]:Objective to investigate the expression of c-cbl in ovarian cancer and its effect on the proliferation of ovarian cancer cells. Methods the expression of c-cbl protein in ovarian cancer tissue was detected by immunohistochemical method and S-P method. The expression of c-cbl protein in ovarian cancer tissue was detected by Edu. The proliferation ability of ovarian cancer SKOV3 cells after silencing c-cbl gene was detected. Transwell was used to detect the invasion ability of ovarian cancer SKOV3 cells. Results c-cbl protein was expressed in the cytoplasm of ovarian cancer. The expression of c-cbl protein was strongly positive in ovarian cancer. The expression of c-cbl protein in ovarian cancer was significantly higher than that in normal ovarian tissue, and the expression of c-cbl protein in ovarian cancer was correlated with FIGO staging (P 0.05), and the expression of c-cbl gene in ovarian cancer SKOV3 cells was silenced after silencing c-cbl gene. After silencing c-cbl gene, the expression of P21 and p53 protein was up-regulated. Conclusion the expression of c-cbl protein is up-regulated in ovarian cancer, and the silencing of c-cbl gene may be related to the up-regulation of P21 and p53 proteins.
【作者单位】：重庆市第五人民医院妇产科;重庆医科大学第二附属医院妇产科;
【基金】：国家自然科学基金资助项目(81172492)
【分类号】：R737.31

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