超声介导正己酰羧甲基壳聚糖载药纳米液滴对卵巢癌细胞的抑制作用

发布时间：2018-03-15 04:27

本文选题：羧甲基壳聚糖　切入点：载药纳米液滴　出处：《中国医学影像技术》2017年06期 　论文类型：期刊论文

【摘要】：目的评价采用正己酰羧甲基壳聚糖制备相变型载阿霉素(DOX)纳米液滴的体外超声显像效果及抑制卵巢癌细胞生长的作用。方法将羧甲基壳聚糖与正己酸酐发生酰化反应合成正己酰羧甲基壳聚糖,采用声振乳化法制备正己酰羧甲基壳聚糖载药纳米液滴,表征其表面形态、粒径、电位;评价其体外超声显像效果,以紫外分光光度计测定药物包封率,采用CCK-8法检测卵巢癌细胞存活率,并进行统计学分析。结果成功制备正己酰羧甲基壳聚糖载药纳米液滴,镜下为大小均匀的球状物,平均粒径为(458.33±43.50)nm,包封率为(52.06±10.14)%。纳米液滴可增强超声显影效果。超声联合载药纳米液滴组的卵巢癌细胞存活率[(62.54±3.60)%]低于DOX组[(75.55±7.21)%]、载药纳米液滴组[(76.18±4.94)%]和超声组[(89.90±0.83)%],差异有统计学意义(P均0.05)。结论超声联合正己酰羧甲基壳聚糖载药纳米液滴可有效抑制卵巢癌细胞生长,在临床诊疗中具有潜在应用价值。
[Abstract]:Objective to evaluate the effect of ultrasonic imaging of phase change doxorubicin (DOX) nanoparticles prepared by hexanoyl carboxymethyl chitosan on ovarian cancer cell growth. Methods Carboxymethyl chitosan was acylated with hexanoic anhydride. Synthesis of n-hexyl carboxymethyl chitosan by reaction, Nano-droplets loaded with n-hexyl carboxymethyl chitosan were prepared by acoustical emulsification method, their surface morphology, particle size and potential were characterized, the effect of ultrasonic imaging in vitro was evaluated, and the encapsulation efficiency was measured by ultraviolet spectrophotometer. CCK-8 assay was used to detect the survival rate of ovarian cancer cells, and the results showed that n-hexyl carboxymethyl chitosan nanoparticles were successfully prepared. The average particle size was 458.33 卤43.50 nm, the encapsulation efficiency was 52.06 卤10.140.The sonographic effect was enhanced by nano-droplets. The survival rate of ovarian cancer cells in the combination of ultrasonic and drug-loaded nano-droplet group [62.54 卤3.60%] was lower than that in DOX group [75.55 卤7.21%], the drug loaded nano-droplet group [76.18 卤4.94 渭%] and the ultrasound group [89.90 卤0.833%], the difference was statistically significant. Conclusion Ultrasound combined with n-hexanoyl carboxymethyl chitosan nanoparticles can effectively inhibit the growth of ovarian cancer cells. It has potential application value in clinical diagnosis and treatment.
【作者单位】：广州医科大学附属第三医院药学部;广州医科大学附属第三医院超声医学科;
【基金】：国家自然科学基金面上项目(81371572) 广东省自然科学基金(2016A030311054) 广州市科信局项目(201607010201)
【分类号】：R445.1;R737.31

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