NTs家族成员及其受体Trk在卵巢癌中的表达及其与生存期的相关性

发布时间：2018-03-15 05:23

  本文选题：卵巢癌　切入点：NTs　出处：《现代妇产科进展》2017年11期 　论文类型：期刊论文

【摘要】：目的:分析神经营养素(NTs)家族成员及其酪氨酸激酶受体(Trk)在卵巢癌中的表达情况及其与患者总生存期(OS)的关系,评价其预后价值。方法:利用KaplanMeier plotter(KM plotter)在线数据库收集卵巢癌患者病理信息,分析NTs家族成员(NGF、BDNF、NT-3、NT-5)及其受体Trk(TrkA、TrkB、TrkC)表达与卵巢癌患者预后及临床病理参数的相关性。收集新鲜卵巢癌组织和癌旁组织各6例,Western blot法检测NTs及Trk蛋白表达。结果:BDNF mRNA高表达与卵巢癌患者较好的总生存期(OS)相关(危险比HR=0.78,95%CI为0.68~0.89,P0.001);NGF、NT-3、NT-5及受体TrkA、TrkB、TrkC表达与卵巢癌患者的总生存期(OS)无关(P0.05)。BDNF mRNA表达与临床分期Ⅰ、Ⅱ及Ⅲ期、病理分级Ⅱ级、组织学分型浆液性癌及TP53野生型的卵巢癌患者的OS密切相关(P0.05)。NGF、NT-3及TrkA表达均与卵巢癌患者的临床病理参数不相关(P0.05)。NT-5表达与病理分级Ⅱ级的卵巢癌患者的OS相关(P0.05)。TrkB及TrkC表达与临床分期Ⅲ期的卵巢癌患者的OS密切相关(P0.05)。Western blot法检测显示,NTs家族中NGF、BDNF、NT-5蛋白在卵巢癌组织中过表达,NT-3蛋白则为低表达;Trk家族中TrkA、TrkB及TrkC在卵巢癌组织中高表达。结论:BDNF表达与卵巢癌患者的预后密切相关,高表达患者的预后更好,可作为潜在的预后指标进行研究;NTs家族其他成员及受体Trk家族均无此预测作用。
[Abstract]:Objective: to investigate the expression of neurotrophin NTs) family and its tyrosine kinase receptor (Trk) in ovarian cancer and its relationship with total survival time (OS). Methods: KaplanMeier plotter(KM plotter-based online database was used to collect pathological information of ovarian cancer patients. To analyze the correlation between the expression of NTs family member BDNFNT-3 NT-5) and its receptor TrktkAK B (TrkBU TrkC5) and the prognosis and clinicopathological parameters of ovarian cancer. The expression of NTs and Trk protein were detected by Western blot in 6 fresh ovarian cancer tissues and 6 paracancerous tissues respectively. Results the expression of NTs and Trk protein was high in the tissues of fresh ovarian cancer and paracancerous tissues. There was no correlation between the expression of mRNA and clinical stage 鈪，

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