二甲双胍对胎儿生长受限小鼠追赶生长时期胰岛素抵抗的干预效果

发布时间：2018-03-15 07:07

本文选题：胎儿生长受限　切入点：二甲双胍　出处：《南方医科大学学报》2017年08期 　论文类型：期刊论文

【摘要】：目的探讨二甲双胍干预胎儿生长受限(FGR)小鼠追赶生长时期胰岛素抵抗的有效性。方法采用孕期低蛋白饮食法建立FGR小鼠模型。FGR组和正常对照组(NC)新生雌鼠自断乳后分别喂予标准饲料(SF)和高脂饲料(HF),不同饲料喂养组又分为二甲双胍灌胃组(MF)和生理盐水灌胃组(NS),即:NC+SF+NS组、NC+SF+MF组、NC+HF+NS组、NC+HF+MF组、FGR+SF+NS组、FGR+SF+MF组、FGR+HF+NS组、FGR+HF+MF组。于仔鼠8周龄开始每组取6只观察阴道开口时间及动情周期变化。第12周在动情间期每组取6只小鼠禁食12 h,测体质量、身长,计算体质量系数(PI),采血取血清进行空腹血糖(FPG)、空腹胰岛素(Fins)、促卵泡生成素(FSH)、抗苗勒管激素(AMH)检测,并计算胰岛素抵抗指数(HOMA-IR)。第12周始每组取6只小鼠,与雄鼠2∶1合笼交配,了解雌鼠生殖能力。组间比较采用单因素方差分析,组间差异采用Fisher精确概率检验,以FGR、HF及MF三个因素为分析指标,采用3×2析因实验分析,确定有无交互作用,以及交互作用的显著性水平。结果(1)析因分析:FGR因素及HF因素对PI指数、Fins、HOMA-IR、阴道开口时间、AMH均有交互相加作用的显著性影响(P0.05);MF因素干预HF因素相关的体质量、PI指数、FPG、Fins、HOMA-IR及动情周期开始时间有显著性意义(P0.05);除身长、FSH外,MF因素干预FGR因素相关的其余观察指标均有显著性意义(P0.05)。(2)组间比较:除体质量外,FGR因素对其余观察指标的影响差异均有统计学意义(均P0.05);除FSH外,HF因素对其余观察指标的影响差异均有统计学意义(均P0.05);除身长、FSH外,MF因素对其余观察指标的影响差异均有统计学意义(均P0.05);另FGR+HF+NS组与NC+SF+NS组、FGR+SF+NS组与FGR+HF+NS组妊娠率的差异有统计学意义(P0.05)。结论 FGR小鼠存在青春期延迟、稀发排卵及成年期胰岛素抵抗,出生后高脂饮食促发FGR小鼠的追赶生长,追赶生长时期的二甲双胍干预对其胰岛素抵抗、生殖内分泌紊乱的改善具有有效性。
[Abstract]:Objective to investigate the effect of metformin on insulin resistance during catch-up growth in FGRmice with fetal growth restriction. Methods FGR mice model. FGR group and normal control group were established by low protein diet during pregnancy. Then they were fed with standard feed SFN and high fat feed HFC respectively. Different feed groups were divided into two groups: metformin group (MFM) and normal saline group (NS group), that is, the control group of normal saline group, the group of normal saline, the group of normal saline, the group of normal saline, the group of normal saline, the group of normal saline, the group of NS, the group of NC SF MF, the group of NC HF, the group of FGR SF, the group of FGR SF, the group of FGR SF, the group of FGR SF. In NS group, FGR HF MF group. At 8 weeks of age, 6 mice in each group were taken to observe the changes of vaginal opening time and estrus cycle. At the 12th week, 6 mice in each group were fasting for 12 hours, and their body mass was measured. Body length, body mass coefficient (BMI), serum samples were collected for the determination of fasting blood glucose (FPG), fasting insulin (FIN), FSH (FSH), and anti-Myelian tube hormone (AMH), and the insulin resistance index (IRI) was calculated. At the 12th week, 6 mice were taken from each group. The reproductive ability of female mice was studied by mating with male 2: 1 cage. Univariate ANOVA was used in the comparison among groups, Fisher accurate probability test was used to test the differences between groups, and 3 脳 2 factorial analysis was used to analyze the three factors FGRG HF and MF. To determine if there is interaction, The significant effect of FGR factor and HF factor on Pi index Finsm HOMA-IRR, vaginal opening time and AMH were analyzed. There were significant differences in HOMA-IR and estrus period between the two groups (P 0.05), and the other observation indexes related to the intervention of FGR with MF factor except body length were significant (P 0.05 n. 2): the effect of FGR on the other indexes except body weight was significant. The differences were statistically significant (all P 0.05, P 0.05, P 0.05, P 0.05, P 0.05, P 0.05, P 0.05, P 0.05, P 0.05, P 0.05, P 0.05 respectively). In addition, there was significant difference in pregnancy rate between FGR HF NS group and NC SF NS group and FGR HF NS group. Conclusion there is a delayed puberty in FGR mice. Rare ovulation and adult insulin resistance, postnatal high-fat diet promoted the catch-up growth of FGR mice. Metformin intervention in catch-up growth period was effective in improving insulin resistance and reproductive endocrine disorder.
【作者单位】：广州军区广州总医院妇产科;
【基金】：广东省自然科学基金(S2012010008707)
【分类号】：R714.5

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