阻断MAPK信号通路对异位子宫内膜间质细胞NF-κB、Bcl-2和p21表达的影响

发布时间：2018-03-16 18:16

本文选题：MAPK信号通路　切入点：异位子宫内膜　出处：《广东医学》2015年06期 　论文类型：期刊论文

【摘要】：目的探讨阻断MAPK信号通路后,异位子宫内膜间质细胞中NF-κB、Bcl-2和p21表达的变化情况及可能的作用机制,为临床提供可能的治疗靶点。方法取腹腔镜检查、诊断刮宫、子宫内膜异位症治疗、慢性盆腔炎、卵巢囊肿、痛经等检查治疗的妇女子宫内膜标本30例,分离、纯化间质细胞,建立体外间质细胞培养模型。分为正常组、异位组和异位阻断组。Real-time PCR检测NF-κB、Bcl-2和p21的转录水平,免疫印迹(Western blot)法检测NF-κB、Bcl-2和p21的蛋白表达水平。结果异位子宫内膜间质细胞分离纯度为99%;异位细胞中NF-κB、Bcl-2和p21的mRNA与蛋白表达均较正常组高;阻断MAPK以后,NF-κB、Bcl-2和p21的表达明显下降。结论阻断MAPK信号通路以后,异位子宫内膜间质细胞NF-κB、Bcl-2和p21的表达均降低,其机制可能与该通路阻断后引起下游基因表达减少和蛋白降解增加有关。
[Abstract]:Objective to investigate the changes of the expression of NF- 魏 B MAPK Bcl-2 and p21 in ectopic endometrial stromal cells after blocking the MAPK signaling pathway, and to provide a possible therapeutic target for clinical use. Methods Laparoscopic examination was performed to diagnose curettage. 30 endometrial specimens of women treated with endometriosis, chronic pelvic inflammatory disease, ovarian cyst and dysmenorrhea were isolated and purified to establish the culture model of stromal cells in vitro. Real-time PCR was used to detect the transcription levels of Bcl-2 and p21 in ectopic group and ectopic blocking group. Western blotting was used to detect the protein expression of NF- 魏 B, Bcl-2 and p21 in ectopic endometrial stromal cells. Results the purity of mRNA and p21 in ectopic endometrial stromal cells was 999.The expression of mRNA and p21 in ectopic endometrial cells was higher than that in normal endometrium. Conclusion after blocking the MAPK signaling pathway, the expression of NF- 魏 Bm-2 and p21 in ectopic endometrial stromal cells decreased significantly. The mechanism may be related to the decrease of downstream gene expression and the increase of protein degradation after blocking the pathway.
【作者单位】：河北北方学院附属第一医院妇产科;
【基金】：河北省张家口市2013年度科学技术研究与发展计划自筹经费项目(编号:1321081D)
【分类号】：R711.71

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