蛋白酶体抑制剂硼替佐米联合顺铂对宫颈癌细胞增殖的影响

发布时间：2018-03-18 03:21

本文选题：蛋白酶体抑制剂　切入点：硼替佐米　出处：《山东大学学报(医学版)》2018年01期 　论文类型：期刊论文

【摘要】：目的观察蛋白酶体抑制剂硼替佐米(BTZ)联合顺铂(CIS)对宫颈癌He La细胞增殖的影响并研究其相关机制。方法将Hela细胞分为NC组、CIS组、BTZ组、CB组,分别以DMEM培养液、含CIS的培养液、含BTZ的培养液、含CIS及BTZ的培养液培养。以CCK-8法检测各组细胞的增殖抑制率,Western blotting法检测各组细胞中p53与Rb蛋白的表达水平,流式细胞仪检测细胞凋亡率。组间比较采用析因设计资料的方差分析。结果CB组细胞增殖抑制率高于其他各组(P0.05)。CIS组及CB组中p53表达量较高(P0.05),BTZ组与NC组相比差异无统计学意义。与NC组相比,BTZ组Rb表达量较高(P0.05),CIS组、CB组与NC组相比,差异无统计学意义。流式结果示CIS和BTZ在诱导细胞凋亡方面具有交互作用。结论 CIS可能通过调控p53通路对宫颈癌He La细胞增殖产生抑制作用,而BTZ可能通过调控Rb通路对宫颈癌He La细胞增殖产生抑制作用,且BTZ与CIS联用在抑制He La细胞增殖方面具有协同交互作用。
[Abstract]:Objective to observe the effect of bortezomil (BTZ), a proteasome inhibitor, and cisplatin cisplatin (CISZ) on the proliferation of cervical cancer He-La cells and to study its mechanism. Methods Hela cells were divided into two groups: DMEM medium and CIS medium. The proliferation inhibition rate of cells in each group was detected by CCK-8 assay and the expression of p53 and RB protein was detected by Western blotting assay. Apoptosis rate was detected by flow cytometry. ANOVA of factorial design data was used in the comparison among groups. Results the inhibition rate of cell proliferation in CB group was higher than that in other groups (P0.05A. CIS group and CB group). The expression of p53 in BTZ group was higher than that in NC group. There was no difference between CB group and NC group. The expression of RB in BTZ group was higher than that in NC group. Flow cytometry showed that CIS and BTZ had interaction in inducing apoptosis. Conclusion CIS may inhibit the proliferation of He-La cells by regulating p53 pathway. BTZ may inhibit the proliferation of He-La cells by regulating RB pathway, and the combination of BTZ and CIS has a synergistic interaction in inhibiting the proliferation of He-La cells.
【作者单位】：山东大学癌症研究中心;
【基金】：国家自然科学基金(81471944)
【分类号】：R737.33

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