DNA损伤诱导的长链非编码RNA-UCA1促进HeLa细胞增殖

发布时间：2018-03-18 14:04

本文选题：长链非编码RNA　切入点：尿路上皮癌抗原(UCA)　出处：《中国生物化学与分子生物学报》2015年05期 　论文类型：期刊论文

【摘要】：尿路上皮癌抗原1(UCA1)是一种长链非编码RNA,在多种肿瘤内高表达.然而,其在宫颈癌细胞和组织中的表达报告颇不一致,且功能尚未确定.本文探索UCA1在宫颈癌He La细胞中的生物学功能.实时定量PCR(qRT-PCR)结果显示,UCA1、p21和p53 mRNA在阿霉素(doxorubicin,DOX)或γ射线照射的He La细胞中表达上调;相反,敲减p53表达则可抑制DOX诱导的UCA1上调.表明DNA损伤诱导的UCA1可能与p53有关.转染结合CCK8检测He La细胞增殖活力结果显示,与对照比较,过表达UCA1促进He La细胞增殖,干扰UCA1表达则减缓细胞增殖.此外,流式细胞术结果显示,过表达UCA1导致阿霉素诱导的凋亡率下降;siRNA抑制UCA1表达后引起细胞G2/M期比例上升,S期下降,且阿霉素诱导的细胞凋亡率上升.上述结果说明,DNA损伤诱导的UCA1可促进He La细胞增殖,减少细胞凋亡.然而,是否DNA损伤诱导的UCA1上调依赖p53尚需进一步实验证明.
[Abstract]:Urothelial carcinoma antigen (UCA1) is a long chain noncoding RNAs that are highly expressed in many tumors. However, the reports of UCA1 expression in cervical cancer cells and tissues are quite inconsistent. In this study, we investigated the biological function of UCA1 in cervical cancer He-La cells. The results of real-time quantitative PCRQRT-PCR showed that the expression of UCA1p21 and p53 mRNA was up-regulated in doxorubicindoxindoxindoxindoxindoxindoxindoxindoxindox (doxorubicindoxindoxindoxindoxindox doxindoxindox doxindo@@. Knockout p53 could inhibit the up-regulation of UCA1 induced by DOX, suggesting that the UCA1 induced by DNA damage might be related to p53. The results of transfection combined with CCK8 showed that overexpression of UCA1 could promote the proliferation of He-La cells. In addition, flow cytometry showed that overexpression of UCA1 resulted in a decrease in apoptosis induced by doxorubicin. After inhibiting the expression of UCA1, the ratio of G _ 2 / M phase increased and S phase decreased. These results suggest that UCA1 induced by DNA damage can promote the proliferation of He-La cells and reduce apoptosis. However, whether the up-regulation of UCA1 induced by DNA damage depends on p53 should be further demonstrated.
【作者单位】：大连大学附属中山医院;军事医学科学院放射与辐射医学研究所;大连大学生命科学与技术学院;
【基金】：国家自然科学基金(No.31270836,No.31470782,No.31370760) 北京市自然科学基金(No.7101007)资助~~
【分类号】：R737.33

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