子痫前期血清代谢组学研究

发布时间：2018-03-18 14:05

本文选题：代谢组学　切入点：子痫前期　出处：《重庆医科大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的探讨子痫前期患者血清代谢指纹的变化以及寻找子痫前期的生物标志物,为阐明潜在的病因和发病机制提供线索,探索应用代谢组学方法诊断子痫前期的价值。方法采用基于超高效液相色谱三重四级杆飞行时间质谱(UPLC/Q-TOF-MS)的代谢组学方法,对37例子痫前期患者和21例正常妊娠妇女血清代谢指纹研究,运用模式识别方法对数据进行分析。结果子痫前期患者血清代谢指纹与正常孕妇相比存在差异,本实验找到子痫前期16个潜在的生物标志物。应用代谢组学方法对子痫前期进行诊断,其灵敏度、特异度和准确率分别为100%、83.3%、94.1%。结论子痫前期患者血清代谢指纹明显异于正常孕妇,代谢标志物变化趋可以揭示子痫前期可能的发病机理.本实验也为子痫前期的诊断提供了一种新方法,该方法具有良好的诊断能力。
[Abstract]:Objective to explore the changes of serum metabolic fingerprints in patients with preeclampsia and to search for biomarkers of preeclampsia so as to provide clues for elucidating the underlying etiology and pathogenesis of preeclampsia. To explore the value of metabonomics in the diagnosis of preeclampsia. Methods Metabolomics based on UPLC / Q-TOF-MS was used. The serum metabolic fingerprints of 37 preeclampsia patients and 21 normal pregnant women were analyzed by pattern recognition method. Results the serum metabolic fingerprints of preeclampsia patients were different from those of normal pregnant women. In this study, 16 potential biomarkers of preeclampsia were found. The sensitivity, specificity and accuracy of the diagnosis of preeclampsia were 100%, 83.3% and 94.1, respectively. Conclusion the serum metabolic fingerprints of preeclampsia patients are significantly different from those of normal pregnant women. The change of metabolic markers may reveal the possible pathogenesis of preeclampsia. This study also provides a new method for the diagnosis of preeclampsia, which has a good diagnostic ability.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R714.244

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