吲哚美辛对宫颈癌Hela细胞增殖和凋亡作用的影响

发布时间：2018-03-19 06:52

本文选题：吲哚美辛　切入点：宫颈癌Hela细胞　出处：《中国药房》2015年31期 　论文类型：期刊论文

【摘要】：目的:研究吲哚美辛对宫颈癌Hela细胞增殖、凋亡的影响。方法:以体外培养的Hela细胞为试验对象,分别用0(空白对照)、200、400、600、800、1 000μmol/L吲哚美辛作用24、48、72 h后,采用MTT法检测吲哚美辛对Hela细胞的增殖抑制率;用0(空白对照)、400、600、800μmol/L吲哚美辛作用24 h后,采用倒置显微镜观察细胞形态的改变,并用流式细胞仪检测细胞周期分布和细胞凋亡情况。结果:600、800、1 000μmol/L吲哚美辛可抑制Hela细胞的增殖,增殖抑制率与药物浓度和作用时间呈正相关。与空白对照比较,吲哚美辛可使Hela细胞形态由多角形逐渐向圆形转变且细胞呈现凋亡和坏死状态;其中细胞G0/G1期比例增加、S期比例减少,细胞凋亡率增加。结论:吲哚美辛可抑制Hela细胞增殖,诱导其凋亡,主要阻滞细胞于G0/G1期。
[Abstract]:Objective: to study the effect of indomethacin on the proliferation and apoptosis of cervical cancer Hela cells. Methods: Hela cells cultured in vitro were treated with indomethacin for 244872 hours. The inhibitory rate of indomethacin on the proliferation of Hela cells was detected by MTT assay, and the morphological changes of Hela cells were observed by inverted microscope after 24 hours of exposure to indomethacin at 600 渭 mol/L. Cell cycle distribution and apoptosis were detected by flow cytometry. Results the proliferation of Hela cells was inhibited by 1 000 渭 mol/L of 1 000 渭 mol/L. The inhibitory rate of proliferation was positively correlated with the concentration and time of action. Indomethacin could gradually change the shape of Hela cells from polygonal to circular and present apoptosis and necrosis, in which the proportion of G _ 0 / G _ 1 phase increased in S phase and the apoptosis rate increased. Conclusion: indomethacin can inhibit the proliferation of Hela cells. Apoptosis was induced, mainly in G _ 0 / G _ 1 phase.
【作者单位】：浙江省人民医院药学部;浙江中医药大学药学院;
【分类号】：R737.33

【参考文献】