Bcl-2和S100A6在不同子宫平滑肌肿瘤中的表达和意义

发布时间：2018-03-19 17:17

本文选题：子宫平滑肌肿瘤　切入点：Bcl-2蛋白　出处：《山西医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的通过检测Bcl-2、S100A6在良性子宫平滑肌瘤、富于细胞性子宫平滑肌瘤、子宫平滑肌肉瘤中的表达及意义，探讨它们在子宫肌瘤发生发展中的作用及与临床病理指标的关系，并可能进一步为临床鉴别诊断和预后提供依据。方法选取良性子宫平滑肌瘤（UL）30例，富于细胞性子宫平滑肌瘤30例，子宫平滑肌肉瘤（LMS）10例，采用免疫组织化学SABC-9000法分析检测Bcl-2、S100A6在30例良性子宫平滑肌瘤（UL），30例富于细胞性子宫平滑肌瘤及10例子宫平滑肌肉瘤（LMS）中的表达，并对其表达特点进行分析。结果 1.Bcl-2阳性反应位于细胞浆，为棕色颗粒：在30例良性平滑肌瘤（UL）中的阳性表达28例（93.3%），在30例富于性平滑肌瘤（CL）中的阳性表达27例（90.0%），在10例子宫平滑肌肉瘤（LMS）中的阳性表达4例（40%）。三组表达率比较有统计学意义（X2=14.228，P=0.018），UL组和CL组比较无统计学差异（X2=0.947，P=0.862），UL组和LMS组比较有统计学差异（X2=12.624，P=0.002），CL组和LMS组比较有统计学差异（X2=9.732，P=0.012）。 2. S100A6阳性反应位于细胞核或细胞浆，为棕色颗粒：在30例良性平滑肌瘤（UL）中的阳性表达9例（30.0%），在30例富于性平滑肌瘤（CL）中的阳性表达17例（56.7%），在10例子宫平滑肌肉瘤（LMS）中的阳性表达9例（90%）。其阳性表达呈递增趋势，三组表达率比较有统计学意义（X2=11.733，P=0.003），UL组和CL组比较有统计学差异（X2=4.344，P=0.037），UL组和LMS组比较有统计学差异（X2=8.620，P=0.003），CL组和LMS组比较有统计学差异（X2=4.596，P=0.032）。 3.Bcl-2、S100A6在UL、CL和LMS中的表达与患者的年龄、肿瘤结节的大小无关（P0.05）。结论 1. Bcl-2在良性子宫平滑肌瘤、富于细胞性子宫平滑肌瘤中的表达远远高于其在子宫平滑肌肉瘤组织中的表达。提示Bcl-2表达阳性率高的可能在抑制肿瘤进展方面起一定的作用，进一步证实富于细胞性子宫平滑肌瘤是良性肿瘤。 2. S100A6在良性子宫平滑肌瘤、富于细胞性子宫平滑肌瘤、子宫平滑肌肉瘤组织中的表达呈递增趋势，S100A6的表达随子宫平滑肌肿瘤恶性程度的增加而增加，说明富于细胞性子宫平滑肌瘤可能是介于两者之间的一种肿瘤，有恶变的倾向，推测S100A6在三种肌瘤的鉴别诊断中有一定的辅助价值。 3. Bcl-2和S100A6的异常表达与恶性肿瘤细胞的增殖和分化有密切的关系，通过检测Bcl-2和S100A6有可能对子宫肌瘤的发生发展以及预后作出一个有益的判断，为临床治疗和判断肌瘤的良恶性、是否保留子宫及判断预后提供了一定的理论依据。
[Abstract]:Objective to investigate the expression and significance of Bcl-2mS100A6 in benign uterine leiomyoma, Cytoleiomyoma and uterine leiomyosarcoma, and to explore their role in the development of uterine leiomyoma and its relationship with clinicopathological indexes. Methods 30 cases of benign uterine leiomyoma, 30 cases of cytosolic leiomyoma, 10 cases of uterine leiomyosarcoma and 10 cases of uterine leiomyosarcoma were selected. Immunohistochemical SABC-9000 method was used to detect the expression of Bcl-2mS100A6 in 30 cases of benign uterine leiomyoma and 10 cases of uterine leiomyosarcoma. The expression characteristics of Bcl-2mS100A6 in 30 cases of benign uterine leiomyoma and 10 cases of uterine leiomyosarcoma were analyzed. Results. 1. Bcl-2 positive reaction was found in cytoplasm, In 30 cases of benign leiomyoma (ULL), 28 cases (93.3%) were positive, 27 cases (90.0%) were positive in 30 cases of leiomyoma, and 4 cases were positive in 10 cases of uterine leiomyosarcoma (LMSs). There was no significant difference between the two groups. There was no statistical difference between the two groups. There was statistical difference between the two groups. There was statistical difference between the two groups. There was statistical difference between the two groups. There was a statistical difference between the two groups. There was a statistical difference between the two groups. There was a statistical difference between the two groups. There was a statistical difference between the two groups (X29.732P0. 012). 2. The S100A6 positive reaction is located in the nucleus or cytoplasm, For brown granules: in 30 cases of benign leiomyoma (ULL), 9 cases (30.0%), 17 cases (56.7%) and 10 cases of uterine leiomyosarcoma (LMS), respectively. The positive expression showed an increasing trend, and the positive expression was 30.0% in 30 cases of leiomyoma, 56.7% in 30 cases of leiomyoma, and 9 cases in LMS of 10 cases of uterine leiomyosarcoma. There was statistical difference between the three groups in the expression rate of X2X2, 11.733, P0. 003, UL and CL. There was a statistical difference between X2X2, 4. 344, P0. 037, UL and LMS. There was statistical difference between the CL group and the LMS group. There was a statistical difference between the two groups (X24.596P0. 032). 3. The expression of Bcl-2mS100A6 in ULTCL and LMS was not related to the age of patients and the size of tumor nodules. Conclusion. 1. The expression of Bcl-2 in benign uterine leiomyoma and Cytoleiomyoma is much higher than that in uterine leiomyosarcoma, suggesting that the high expression rate of Bcl-2 may play a role in inhibiting the progression of uterine leiomyoma. It is further confirmed that Cytoleiomyoma is a benign tumor. 2. The expression of S100A6 in benign uterine leiomyoma and Cytoleiomyoma was increased with the increase of malignant degree of uterine leiomyosarcoma, and the expression of S100A6 in uterine leiomyosarcoma increased with the increase of malignant degree of uterine leiomyosarcoma. It is suggested that Cytoleiomyoma may be a kind of tumor between them, and it is likely that S100A6 may be helpful in the differential diagnosis of the three kinds of leiomyomas. 3. The abnormal expression of Bcl-2 and S100A6 is closely related to the proliferation and differentiation of malignant tumor cells. The detection of Bcl-2 and S100A6 may make a useful judgment on the occurrence, development and prognosis of uterine leiomyoma. It provides a theoretical basis for clinical treatment and judgment of benign and malignant leiomyoma, whether to preserve uterus and to judge prognosis.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

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