IL-18基因多态性与原因不明复发性流产的关系

发布时间：2018-03-20 13:12

本文选题：不明原因复发性流产　切入点：白细胞介素-18　出处：《浙江大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的探讨人白细胞介素-18(IL-18)基因rs187238(-137G/C).rs360718(-119A/C)、 rs360717(-105G/A)位点多态性与不明原因复发性流产的关系。方法选取207例不明原因复发性流产患者和144例健康妊娠妇女进行研究。通过聚合酶链反应(PCR)联合DNA测序技术分析IL-18基因多态性。结果不明原因复发性流产组rs187238(-137G/C)位点GG.GC+CC基因型分布频率分别为77.3%、22.7%,与对照组GG.GC+CC基因型分布频率(95.8%、4.2%)比较,差异有统计学意义(χ2=22.767,P=0.000)；不明原因复发性流产组rs187238(-137G/C)位点C等位基因频率为13.04%,与对照组C等位基因频率2.1%比较,差异亦有统计学意义(χ2=26.102,P=0.000)；等位基因频率的相对风险分析发现,C等位基因携带者其自然流产的风险是G等位基因携带者的7.050倍(OR=7.050CI：2.990～16.622)。rs360718和rs360717位点基因型分布与对照组均无统计学意义(χ2值分别为1.497和0.858,P值分别为0.221和0.354)；rs360718和rs360717位点两组间等位基因分布频率的差异均无统计学意义(P0.05)。结论 IL-18基因启动子rs187238(-137G/C)位点GC+CC基因型可能是复发性流产易感基因型,C等位基因可能是不明原因复发性流产发病的遗传易感基因之一。IL-18基因外显子rs360718、rs360717位点多态性与原因不明复发性流产可能无相关性。
[Abstract]:Purpose. To investigate the relationship between the polymorphism of rs187238- 137G / CU. Rs360718A- 119A / C, rs360717- 105G- 105G/ A) in human interleukin-18 (IL-18) gene and unexplained recurrent abortion. Method. The polymorphism of IL-18 gene was analyzed by polymerase chain reaction (PCR) and DNA sequencing in 207 patients with unexplained recurrent abortion and 144 healthy pregnant women. The distribution frequency of GG.GC CC genotype at rs187238- 137G / C) locus in recurrent abortion group with unknown cause was 77.3% and 22.7g, respectively, compared with that of control group (95.8%). The frequency of C allele at rs187238- 137G / C was 13.04g / C, which was higher than that of control group (2.1%), and the difference was statistically significant (蠂 ~ 2 / 22.767) (蠂 ~ (2 / 2) = 22.767), and the frequency of C allele was 13.04g / C in the group of recurrent abortion with unknown cause. The relative risk analysis of allelic frequency showed that the risk of spontaneous abortion was 7.050 times that of G allele carriers, and the genotype distribution of Rs360718 and rs360717 loci in G allele carriers was 7.050 times higher than that in G allele carriers. Neither the genotype distribution of Rs360718 nor that of the control group was normal. There was no significant difference in allele frequencies between the two groups (蠂 ~ 2 = 1.497 and 0.858g P = 0.221 and 0.354rs360718 and rs360717, respectively). Conclusion. IL-18 promoter rs187238-137G / C genotype GC CC genotype may be one of the genetic susceptibility genes for recurrent abortion with unknown cause. Polymorphism and causes of rs360718rs360717 gene exon rs360718rs360717 may be one of the genetic susceptibility genes of recurrent abortion with unknown cause. Unknown recurrent abortion may not be related.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.21

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