子痫前期患者胎盘组织中visfatin蛋白与mRNA表达及意义

发布时间：2018-03-20 18:03

本文选题：子痫前期　切入点：胎盘　出处：《中国病理生理杂志》2015年02期 　论文类型：期刊论文

【摘要】：目的:探讨内脂素(visfatin,VF)在子痫前期(PE)患者胎盘组织中的表达及意义。方法:选择2011年8月至2013年12月在温州医科大学附属第一医院住院分娩的孕妇共计100例,根据病情轻重分为重度PE组、轻度PE组和正常妊娠组。采用苏木素-伊红染色法观察3组胎盘组织病理变化;免疫组化法及real-time PCR技术分别检测3组胎盘VF蛋白及mRNA的表达并分析其与子痫前期发病的关系。结果:PE的病理改变主要表现为细胞滋养细胞及合体滋养细胞结构紊乱且形态不完整;细胞滋养细胞增生,合体滋养细胞结节增多;绒毛毛细血管减少、淤血。免疫组化及real-time PCR结果显示胎盘组织中内脂素定位于合体及细胞滋养层细胞的胞浆,随着病情的加重,VF蛋白及mRNA表达量逐渐升高,重度PE组明显高于正常妊娠组,差异有统计学意义(P0.05)。结论:PE患者存在绒毛血管内皮细胞损伤和功能紊乱。胎盘VF蛋白及mRNA在PE孕妇中高表达,表明VF与PE的发生具有一定关系。
[Abstract]:Objective: to investigate the expression and significance of visfatin VFF in placenta of preeclampsia patients with PE.Methods: from August 2011 to December 2013, 100 pregnant women were hospitalized and delivered in the first affiliated Hospital of Wenzhou Medical University. According to the severity of the disease, they were divided into three groups: severe PE group, mild PE group and normal pregnancy group. The pathological changes of placenta were observed by hematoxylin eosin staining method. Immunohistochemical method and real-time PCR technique were used to detect the expression of VF protein and mRNA in placenta of three groups and to analyze the relationship between VF protein and preeclampsia. Results the pathological changes of 10% PE were mainly cytotrophoblast and syncytiotrophoblastic structure. Disorder and incomplete form; Cytotrophoblast proliferation, syncytiotrophoblastic nodule increase, chorionic capillary decrease, congestion. Immunohistochemical and real-time PCR results showed that endolipidins in placenta were located in cytoplasm of syncytiotrophoblast and cytotrophoblastic cells. With the exacerbation of the disease, the expression of VF protein and mRNA increased gradually, and the expression of VF protein in severe PE group was significantly higher than that in normal pregnancy group. The difference was statistically significant (P 0.05). Conclusion VF and mRNA are highly expressed in the placenta of pregnant women with PE, suggesting that VF has a certain relationship with the occurrence of PE.
【作者单位】：温州医科大学附属第一医院产科;温州医科大学附属第一医院外科实验室;温州医科大学附属第一医院病理科;
【基金】：温州市科技局资助项目(No.Y20110256)
【分类号】：R714.244

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