胡桃醌对人宫颈癌HeLa细胞侵袭与迁移的影响

发布时间：2018-03-22 18:16

本文选题：胡桃醌　切入点：HeLa细胞　出处：《解剖学报》2017年02期 　论文类型：期刊论文

【摘要】：目的观察胡桃醌对HeLa细胞侵袭及迁移的抑制作用,并探讨其可能的作用机制。方法常规体外培养人HeLa细胞,加入10、20、50、100μmol/L浓度的胡桃醌,继续培养24 h。通过倒置显微镜观察细胞形态学变化,采用MTT法检测细胞增殖情况,细胞划痕实验检测胡桃醌对细胞迁移能力的影响,用Transwell小室法检测细胞侵袭能力的影响。Western blotting检测基质金属蛋白酶(MMP)-2、MMP-9蛋白的表达。结果细胞形态学观察显示,不同浓度胡桃醌均可导致细胞形态学发生改变;MTT检测表明,胡桃醌对HeLa细胞的生长有明显抑制作用,且抑制作用随药物浓度的增加而增强(P0.05或P0.01);划痕实验结果显示,随胡桃醌药物浓度的增加,细胞的平面运动能力明显下降;Transwell侵袭小室实验结果显示,胡桃醌对HeLa细胞体外的侵袭力具有很强的抑制作用;Western blotting结果表明,胡桃醌给药能够抑制HeLa细胞MMP-2、MMP-9蛋白的表达。结论胡桃醌能够抑制HeLa细胞的增殖并抑制细胞侵袭,其机制可能与抑制MMP-2、MMP-9的表达有关。
[Abstract]:Objective to observe the inhibitory effect of walnut quinone on the invasion and migration of HeLa cells and to explore its possible mechanism. The morphological changes of cells were observed by inverted microscope, cell proliferation was detected by MTT assay, and the effect of walnut quinone on cell migration was detected by cell scratch assay. Transwell chamber assay was used to detect the cell invasion ability. Western blotting was used to detect the expression of matrix metalloproteinase MMP-2MMP-2MMP-9 protein. The growth of HeLa cells was inhibited by nutanone, and the inhibitory effect increased with the increase of drug concentration (P 0.05 or P 0.01), and the scratch test showed that the inhibitory effect increased with the increase of drug concentration of pectin. The results of transwell invasive chamber experiments showed that walnut quinone had a strong inhibitory effect on the invasion of HeLa cells in vitro. Conclusion Nutanone can inhibit the proliferation and invasion of HeLa cells, and its mechanism may be related to the inhibition of MMP-2 MMP-9 expression.
【作者单位】：吉林医药学院生理学教研室;
【基金】：国家自然科学基金(21102055) 吉林省科技厅科技发展项目(20130101157JC) 吉林省教育厅“十二五”科学技术研究项目(2012-334);吉林省教育厅“十二五”科学技术研究项目(2014-363)
【分类号】：R737.33

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