荷瘤小鼠卵巢癌细胞miRNA和髓源性抑制细胞的检测及相关性分析

发布时间：2018-03-23 23:05

本文选题：微小RNA　切入点：髓源性抑制细胞　出处：《细胞与分子免疫学杂志》2015年04期

【摘要】：目的检测卵巢癌荷瘤小鼠癌组织miR-21-5p、miR-155-5p、miR-218-5p、miR-222-3p、miR-494-3p的水平和外周血和脾脏中髓源性抑制细胞(MDSC)的数量,并分析它们的相关性。方法 12例卵巢癌荷瘤小鼠的癌组织和癌旁组织,实时荧光定量PCR检测癌组织和癌旁组织中miR-21a-5p、miR-155a-5p、miR-218-5p、miR-222-3p、miR-494-3p的水平,流式细胞术检测上述荷瘤小鼠中外周血和脾脏中MDSC的表达。采用Spearman法分析MDSC和microRNA的相关性。结果与正常小鼠相比,荷瘤小鼠外周血和脾脏中的MDSC明显增加。miR-21a-5p、miR-218-5p和miR-222-3p在癌组织中含量高于癌旁组织,相反miR-155a-5p和miR-494-3p在癌组织中的表达低于癌旁组织。miR-222-3p与MDSC的数量无相关性,miR-494-3p的水平与MDSC的数量负相关,miR-21a-5p、miR-155a-5p、miR-218-5p在癌组织与癌旁组织的表达差值与MDSC的数量呈正相关。结论卵巢癌荷瘤小鼠癌组织中miR-21a-5p、miR-155a-5p、miR-218-5p、miR-494-3p水平和外周血及脾脏中MDSC的数量有一定的相关性。
[Abstract]:Objective to detect the level of miR-21-5pnmiR-155-5pnmiR-218-5pnmiR-222-3pmmiR-494-3p and the number of myelogenous suppressor cells (MDSCs) in peripheral blood and spleen of mice bearing ovarian cancer, and to analyze their correlation. Methods the tumor tissues and adjacent tissues of 12 cases of ovarian cancer bearing mice were studied. Real-time fluorescence quantitative PCR was used to detect the level of miR-21a-5pnmiR-155a-5pnmiR-218-5pnmiR-222-3pmmiR-494-3p, flow cytometry was used to detect the expression of MDSC in peripheral blood and spleen of the above tumor-bearing mice, and the correlation between MDSC and microRNA was analyzed by Spearman method. Results compared with normal mice, the expression of MDSC in peripheral blood and spleen was detected by flow cytometry. The levels of MDSC and miR-222-3p in peripheral blood and spleen of tumor-bearing mice were significantly higher than those in para-cancerous tissues, and the contents of .miR-21a-5pmmiR-218-5p and miR-222-3p in cancer tissues were significantly higher than those in paracancerous tissues. In contrast, the expression of miR-155a-5p and miR-494-3p in cancer tissues was lower than that in paracancerous tissues. There was no correlation between the expression of miR-222-3p and the number of MDSC. There was a negative correlation between the level of miR-494-3p and the number of MDSC. There was a positive correlation between the expression difference of miR-21a-5pnmmiR-155a-5ptimiR-218-5p and the quantity of MDSC. The levels of miR-21a-5pnmiR-155a-5pnmiR-218-5pnmiR-494-3p were correlated with the number of MDSC in peripheral blood and spleen of tumor-bearing mice.
【作者单位】：江苏大学第四附属医院;江苏大学免疫学研究所;
【基金】：江苏省六大人才高峰项目(2013-WSN-002) 江苏省临床医学专项(BL2012059) 江苏省妇幼课题(F201350)
【分类号】：R737.31

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