CTLA-4基因多态性与宫颈癌易感性的关系

发布时间：2018-03-25 05:21

本文选题：宫颈癌　切入点：细胞毒T淋巴细胞相关抗原　出处：《中国老年学杂志》2017年17期

【摘要】：目的探讨细胞毒T淋巴细胞相关抗原(CTLA)-4基因单核苷酸多态性(SNPs)与宫颈癌发生的关系。方法 350例宫颈癌患者为病例组,350例健康女性为对照组,抽取全血基因组DNA,直接测序法检测CTLA-4-318 T/C、CT60 G/A单核苷酸多态性,采用MTT法检测不同基因型的外周血单核细胞增殖能力,酶联免疫吸附(ELISA)法检测不同基因型患者血清转化生长因子(TGF)-β、白细胞介素(IL)-2、IL-4表达水平。结果宫颈癌病例组CTLA-4-318位点等位基因C的概率低于对照组,等位基因T概率高于对照组,CC基因型比例低于对照组,TT基因型比例高于对照组(均P0.05)。病例组CTLA-4 CT60位点等位基因A的概率低于对照组,等位基因G的概率高于对照组,AA基因型比例低于对照组,GG基因型比例高于对照组(均P0.05)。CTLA-4-318 CC基因型T细胞增殖率高于TT基因型,TGF-β水平低于TT基因型,IL-2、IL-4水平高于TT基因型(均P0.05)。CTLA-4 CT60 AA基因型TGF-β水平低于GG基因型,IL-2、IL-4水平高于GG基因型(P0.05)。结论 CTLA-4-318 T/C、CT60 G/A基因多态性在宫颈癌的发生发展过程中起到重要作用,CTLA-4-318 TT基因型及CT60 GG基因型可增加宫颈癌的发病风险,其机制可能与调节T细胞的增殖及调节细胞因子的分泌有关。
[Abstract]:Objective to investigate the relationship between cytotoxic T lymphocyte associated antigen (CTLA-4) single nucleotide polymorphisms (SNPs) and cervical carcinogenesis. The genomic DNA of whole blood was extracted, and the single nucleotide polymorphisms of CTLA-4-318 T / CT60 G / A were detected by direct sequencing. The proliferative ability of peripheral blood monocytes with different genotypes was detected by MTT method. The levels of TGF- 尾 and IL-4 in serum of patients with different genotypes were detected by Elisa. Results the probability of allele C at CTLA-4-318 locus in cervical cancer patients was lower than that in control group. The probability of allele T was higher than that of control group (P < 0.05). The probability of allele A at CTLA-4 CT60 locus in case group was lower than that in control group. The probability of allele G was higher than that of the control group. The percentage of AA genotype was lower than that of the control group. The percentage of T cell proliferation of P0.05).CTLA-4-318 CC genotype was higher than that of the control group. The level of TGF- 尾 was lower than that of TGF- 尾 of TT genotype and the level of TGF- 尾 was higher than that of TT genotype. The level of TGF- 尾 of P0.05).CTLA-4 CT60 AA genotype was lower than that of GG genotype (P 0.05). Conclusion the polymorphism of CTLA-4-318 T / CfCT60 G / A gene plays an important role in the occurrence and development of cervical cancer. CTLA-4-318TT genotype and CT60 GG genotype may play an important role in the occurrence and development of cervical cancer. Increase the risk of cervical cancer, The mechanism may be related to the regulation of T cell proliferation and cytokine secretion.
【作者单位】：海南省农垦三亚医院妇产科;
【分类号】：R737.33

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