多囊卵巢综合征痰湿证与脂联素及脂联素受体蛋白表达的相关研究

发布时间：2018-03-26 11:55

本文选题：多囊卵巢综合征　切入点：痰湿证　出处：《中华中医药学刊》2015年01期

【摘要】：目的:通过分析多囊卵巢综合征(PCOS)痰湿证患者血清和卵泡液脂联素(APN)水平及卵巢黄素化颗粒细胞脂联素受体(AdipoR1/AdipoR2)蛋白表达的差异,从卵巢局部分子水平探讨PCOS痰湿证的发病机理。方法:选择行体外受精-胚胎移植(IVF-ET)的PCOS患者共43例(痰湿证20例,非痰湿证23例)及对照组30例,化学发光法检测三组患者血清胰岛素(FIN)水平;取卵日留取外周血及卵泡液,并提取黄素化颗粒细胞,酶联免疫吸附法(ELISA)测定血清和卵泡液中脂联素(APN)水平,Western blot法测定黄素化颗粒细胞脂联素受体蛋白表达水平。结果:1各组间一般情况及血清激素水平的比较:PCOS痰湿组BMI及FIN值明显升高(P0.01);与对照组相比,PCOS两组LH及T值升高(P0.01),PCOS痰湿组FSH值降低(P0.01);PCOS痰湿组LH值低于非痰湿组(P0.05);各组年龄及PRL和E2值差异无统计学意义(P0.05);2PCOS痰湿组血清及卵泡液APN水平、AdipoR1/AdipoR2蛋白表达水平均显著性低于余两组(P0.05)。3血清APN、卵泡液APN与BMI及FIN分别呈负相关;BMI与FIN呈正相关。结论:PCOS痰湿证具有明显的胰岛素抵抗特征;其胰岛素抵抗(IR)可能与卵巢局部脂联素受体蛋白表达下降有关。
[Abstract]:Objective: to analyze the difference of serum and follicle fluid adiponectin (APN) levels and adiponectin receptor (AdipoR1 / AdipoR2) expression in patients with polycystic ovary syndrome (PCOS) with phlegm dampness syndrome. To explore the pathogenesis of phlegm dampness syndrome of PCOS at the local molecular level of ovary. Methods: 43 cases of PCOS (20 cases of phlegm dampness syndrome, 23 cases of non-phlegm dampness syndrome) and 30 cases of control group were selected for in vitro fertilization and embryo transfer IVF-ETs. The levels of insulin in the serum of the three groups were detected by chemiluminescence assay, the peripheral blood and follicular fluid were collected on the day of egg taking, and the luteinized granulosa cells were extracted. Enzyme linked immunosorbent assay (Elisa) for the determination of adiponectin (APN) levels in serum and follicular fluid; Western blot assay for the expression of adiponectin receptor protein in luteinized granulosa cells. Compared with the control group, the levels of LH and T in the phlegm wet group were significantly higher than those in the control group. The FSH value in the phlegm wet group was lower than that in the non-phlegm wet group, but there was no significant difference in the age, PRL and E2 values in the serum and the serum and the level of E 2 in the phlegm wet group. The expression of AdipoR1 / AdipoR2 protein in follicular fluid was significantly lower than that in the other two groups (P 0.05), and APN in follicular fluid was negatively correlated with BMI and FIN, respectively. Insulin resistance (IRR) may be associated with decreased adiponectin receptor protein expression in the ovary.
【作者单位】：山东中医药大学;
【基金】：国家自然科学基金青年基金项目(81102560) 山东省自然科学基金青年基金项目(ZR2011HQ050)
【分类号】：R711.75

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