神经生长因子和血管内皮生长因子在上皮性卵巢癌中表达及意义研究

发布时间：2018-03-28 05:20

本文选题：神经生长因子　切入点：血管内皮生长因子　出处：《中国实用妇科与产科杂志》2017年09期

【摘要】：目的探讨神经生长因子(NGF)和血管内皮生长因子(VEGF)在上皮性卵巢癌中的表达及相关性。方法选择2012-2015年在杭州市第一人民医院行手术切除并经病理确诊为上皮性卵巢癌标本60例设为研究组,19例正常卵巢组织设为对照组,使用免疫组织化学法分别检测两组中NGF及VEGF的表达情况;构建NGF shRNA表达载体,使其能够抑制细胞内NGF基因表达,将经NGF shRNA基因下调后的细胞株与阴性对照细胞株(NC)、正常细胞株(normal)进行对照实验,通过Western-blot分别检测干扰前后卵巢癌细胞系A2780中NGF及VEGF蛋白质的表达情况。结果研究组NGF及VEGF蛋白阳性率均为100%,高于对照组中NGF及VEGF蛋白阳性率74%、84%,差异有统计学意义(P=0.004、0.027)。Western-blotting检测到NGF shRNA细胞表达株中NGF、VEGF蛋白水平的抑制率分别为40%、38%。NGF shRNA组中NGF与VEGF蛋白表达显著相关,差异有统计学意义。结论 NGF及VEGF对上皮性卵巢癌的发生、增殖及侵袭等有一定促进作用。
[Abstract]:Objective to investigate the expression and correlation of NGF and VEGF in epithelial ovarian cancer. Methods the epithelial ovary was surgically resected from 2012 to 2015 in the first people's Hospital of Hangzhou and pathologically diagnosed as epithelial ovary. Sixty cases of cancer were set up as study group and 19 cases of normal ovarian tissue as control group. The expression of NGF and VEGF in the two groups was detected by immunohistochemical method, and the expression vector of NGF shRNA was constructed to inhibit the expression of NGF gene in the cells. NGF shRNA down-regulated cell line and negative control cell line, normal cell line, were used in the control experiment. The expression of NGF and VEGF protein in ovarian cancer cell line A2780 was detected by Western-blot. Results the positive rate of NGF and VEGF protein in study group was 100, which was higher than that of NGF and VEGF protein in control group. The difference was statistically significant. The inhibition rate of NGF shRNA protein expression by Western-blotting was 40%. The expression of NGF and VEGF protein in NGF shRNA group was significantly correlated with that in NGF shRNA group. Conclusion NGF and VEGF can promote the occurrence, proliferation and invasion of epithelial ovarian cancer.
【作者单位】：南京大学附属杭州医院(杭州市第一人民医院);
【基金】：浙江省自然科学基金(LY12H16018)
【分类号】：R737.31

【相似文献】