血管内皮生长因子基因与习惯性流产的相关性

发布时间：2018-03-30 08:48

本文选题：习惯性流产　切入点：血管内皮生长因子基因　出处：《西安交通大学学报(医学版)》2015年04期

【摘要】：目的探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)基因10个单核苷酸多态性(single nucleotide polymorphism,SNP)位点与习惯性流产的相关性。方法严格按照诊断标准,选取无亲缘关系的习惯性流产患者241名(习惯性流产组)及健康体检者265名(正常对照组);采取静脉血,提取基因组DNA,采用基质辅助激光解吸电离飞行时间质谱技术检测10个SNP的基因型;采用SPSS11.5及Haploview 4.2软件统计分析基因型、等位基因及单倍型频率在病例组和对照组中的差异。结果 VEGF基因rs3025020(内含子6)及rs3025039(3′非翻译区)基因型及等位基因频率分布在习惯性流产组和正常对照组中的差异有统计学意义(P0.05)。习惯性流产组rs3025020位点T等位基因频率显著高于正常对照组(P=0.000 2,OR=1.620,95%CI=1.260~2.084);习惯性流产组rs3025039位点T等位基因频率显著高于正常对照组(P=0.001,OR=1.628,95%CI=1.209~2.192)。连锁不平衡分析发现4个单倍型高度连锁(D′0.9)。习惯性流产组A-C单倍型(rs833069-rs3024998)频率显著低于正常对照组(P=0.034);习惯性流产组T-G-C单倍型(rs3025020-rs3025030-rs3025039)频率显著低于正常对照组(P=0.032);习惯性流产组C-G-C单倍型(rs3025020-rs3025030-rs3025039)频率显著高于正常对照组(P=0.0002)。结论 VEGF基因rs3025020、rs3025039位点可能与习惯性流产有关,携带有C-G-C单倍型(rs3025020-rs3025030-rs3025039)的个体可能更容易患习惯性流产。
[Abstract]:Objective To investigate the correlation between the polymorphism of vascular endothelial growth factor ( VEGF ) gene and habitual abortion . Methods : 241 patients with habitual abortion ( habitual abortion group ) and 265 healthy controls ( normal control group ) were selected according to the criteria of diagnosis . The frequencies of genotype and allele frequencies of rs3025020 ( intron 6 ) and rs3025039 ( 3 ' untranslated region ) in habitual abortion group were significantly higher than those in normal control group ( P = 0.000 2 , OR = 1.620 , 95 % CI = 1.260 - 2.084 ) . The frequency of rs3025020 - rs3025030 - rs3025039 in habitual abortion group was significantly lower than that of the normal control group ( P = 0.032 ) . Conclusion The rs3025020 and rs3025030 - rs3025039 of habitual abortion group may be associated with habitual abortion , and individuals carrying C - G - C haplotype ( rs3025020 - rs3025030 - rs3025039 ) may be more likely to suffer from habitual abortion .

【作者单位】：温州医科大学附属第二医院妇产科;温州市中西医结合医院;
【分类号】：R714.21

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