Hsa-miR-127-3p在宫颈癌组织中的表达及对宫颈癌细胞迁移和侵袭能力的影响

发布时间：2018-03-31 23:06

本文选题：miR--p　切入点：宫颈癌　出处：《现代妇产科进展》2017年07期

【摘要】：目的:研究hsa-mi R-127-3p(mi R-127)在宫颈癌组织中的表达水平及其与临床病理特征之间的相关性,探讨mi R-127对宫颈癌细胞株Hela及Siha迁移和侵袭的影响。方法:Real-time PCR法检测50对宫颈癌及癌旁组织中mi R-127表达水平,分析mi R-127表达与临床病理特征的相关性。细胞转染mi R-127 mimics及mimics negative control,采用划痕愈合实验检测细胞迁移能力,Transwell法检测细胞侵袭能力。结果:mi R-127在宫颈癌组织中的表达明显低于癌旁组织(P0.05)。mi R-127表达与淋巴脉管间隙浸润相关(P=0.048),而与年龄、FIGO分期、病理类型、分化程度、病灶大小、浸润深度、有无淋巴结转移无关(P0.05)。过表达mi R-127可抑制Hela及Siha细胞的迁移和侵袭能力。结论:mi R127在宫颈癌组织中低表达,并且抑制宫颈癌细胞株Hela及Siha的迁移和侵袭,可能与宫颈癌的发生发展密切相关。
[Abstract]:Objective: to study the expression of hsa-mi R-127-3p(mi R-127 in cervical carcinoma and its correlation with clinicopathological features. To investigate the effect of miR-127 on migration and invasion of cervical cancer cell line Hela and Siha. The relationship between the expression of miR-127 and clinicopathological features was analyzed. The cell transfected mi R-127 mimics and mimics negative control were transfected. The migration ability of cells was detected by scratch healing test and the cell invasion ability was detected by Transwell method. Results the expression of miR-127 was detected in cervical carcinoma tissues. The expression of P0.05Mi R-127 in paracancerous tissue was significantly lower than that in paracancerous tissue and associated with lymphatic vascular interstitial invasion (P0.048), but with age and Figo stage. Pathological type, degree of differentiation, size of lesion, depth of invasion, lymph node metastasis were not related to P0.05.overexpression of miR-127 could inhibit the migration and invasion of Hela and Siha cells. The inhibition of migration and invasion of cervical cancer cell line Hela and Siha may be closely related to the occurrence and development of cervical cancer.
【作者单位】：中山大学孙逸仙纪念医院妇科肿瘤;
【基金】：国家自然基金项目(No:81572575) 广东省自然基金重点项目项目(No:S2012020010913);广东省自然基金项目(No:2014A030313109) 广东省科技计划项目项目(No:2013B21800234)
【分类号】：R737.33

【相似文献】