MiR-886-5p对宫颈鳞状上皮细胞克隆形成和宫颈癌细胞化学药物治疗的影响

发布时间：2018-04-01 17:40

本文选题：宫颈鳞状上皮细胞　切入点：宫颈癌　出处：《首都医科大学学报》2015年06期

【摘要】：目的探讨microRNA-886-5p(MiR-886-5p)对宫颈鳞状上皮细胞克隆形成及宫颈癌细胞化学药物治疗(以下简称化疗)的影响。方法应用基因芯片技术检测宫颈癌及其癌周组织microRNA的表达,筛选出MiR-886-5p在宫颈癌组织中高表达。生物信息系技术分析发现miRNA-886-5p靶向P53通路中多个基因的表达。用MiR-886-5p mimics和带有GFP标签的MiR-886-5p过表达载体稳定转染高危型人乳头瘤病毒(human papilomavirus,HPV16)阳性的永生化人宫颈鳞状上皮细胞H8细胞,应用Western blotting方法检测P53和P14的蛋白表达情况;应用平板克隆形成技术,观察对细胞克隆形成的影响。在宫颈癌Si Ha细胞中,加入化疗药物紫杉醇和VP16后,应用real time RT-PCR技术,检测MiR-886-5p的表达情况。结果过表达MiR-886-5p后,H8细胞的克隆形成率明显高于阴性对照组,并且降调P53通路中P14和P53蛋白的表达。加入化疗药紫杉醇和VP16后,Si Ha细胞中MiR-886-5p表达明显升高。结论 MiR-886-5p与宫颈鳞状细胞增生相关,它降调P14和P53两种蛋白的表达,且与宫颈癌细胞化疗抵抗相关。
[Abstract]:Objective to investigate the effect of microRNA-886-5p (MiR-886-5p) of cervical squamous cell clone formation and cervical cancer cells chemotherapy (hereinafter referred to as chemotherapy). The effects of expression of cervical cancer and its surrounding tissues by using the microRNA method of gene chip technology, screened the high expression of MiR-886-5p in cervical cancer tissues. The analysis found that miRNA-886-5p targeted gene expression in the P53 pathway. The biological information technology department of over expression vector transfected human papilloma virus with MiR-886-5p mimics and GFP tagged MiR-886-5p (human Papilomavirus HPV16) positive immortalized human cervical epithelial cells and H8 cells, Western expression by blotting method to detect P53 and P14 protein; application of colony formation to observe the effect of technology, on the formation of cell clones. Si Ha in cervical cancer cells, with the chemotherapy drug paclitaxel and VP16, should be Using real time RT-PCR technique to detect the expression of MiR-886-5p. Results the expression of MiR-886-5p, H8 cell clone formation rate was significantly higher than that of the negative control group, the expression of P14 and down-regulation and P53 protein in P53 pathway. Adding chemotherapy drug taxol and VP16, significantly increased the expression of MiR-886-5p Si in Ha cells. Conclusion MiR-886-5p and cervical squamous cell hyperplasia, the expression of P14 and P53. Its two proteins, and cervical cancer cell resistance to chemotherapy.

【作者单位】：首都医科大学附属北京天坛医院妇产科;首都医科大学附属北京佑安医院妇产科;
【基金】：国家自然科学基金(81101969)~~
【分类号】：R737.33

【参考文献】