黏附分子CD44v6、ICAM-1与早期宫颈鳞癌淋巴结转移的关系

发布时间：2018-04-02 19:10

本文选题：宫颈肿瘤　切入点：淋巴结转移　出处：《重庆医学》2017年22期

【摘要】：目的探讨黏附分子CD44v6、细胞黏附分子-1(ICAM-1)与早期宫颈鳞癌淋巴结转移的关系。方法收集江西省妇幼保健院宫颈癌Ⅰb1期患者宫颈标本74份,正常宫颈标本20份,宫颈鳞状细胞原位癌标本20份,采用real-time PCR法及免疫组织化学法测定宫颈组织标本CD44v6和ICAM-1的表达,免疫组织化学法测定D2-40标记的淋巴管密度(LVD),并探讨三者与宫颈癌组织分化程度及淋巴结转移的关系。结果 CD44v6及ICAM-1在正常宫颈、宫颈鳞状细胞原位癌、宫颈癌组织中的阳性表达率逐渐提高,分别为0、75.00%、87.84%及10.00%、45.00%、81.08%;mRNA表达水平也逐渐提高,分别为0、0.24±0.02、1.02±0.11及0.10±0.00、0.19±0.02、1.03±0.10,差异均有统计学意义(P0.01)。正常宫颈、宫颈鳞状细胞原位癌、宫颈癌组织中LVD逐渐提高(P0.01)。低分化宫颈癌组织中CD44v6、ICAM-1阳性表达率及LVD高于高、中分化(P0.01),有淋巴结转移宫颈癌组织中CD44v6、ICAM-1阳性表达率及LVD高于无淋巴结转移(P0.01)。宫颈癌组织中CD44v6、ICAM-1及LVD呈两两正相关。结论 CD44v6在宫颈癌进展中起促进作用,与ICAM-1及LVD协同促进宫颈癌发展,可作为判断宫颈癌淋巴结转移及诊断的有效指标。
[Abstract]:Objective to investigate the relationship between adhesion molecule CD44v6 and cell adhesion molecule-1 (ICAM-1) and lymph node metastasis in early cervical squamous cell carcinoma (SCC).Methods 74 cervical specimens, 20 normal cervix specimens and 20 cervical squamous cell carcinoma in situ specimens were collected from cervical cancer patients in Jiangxi Maternal and Child Health Hospital.The expression of CD44v6 and ICAM-1 in cervical tissues was detected by real-time PCR method and immunohistochemical method, and the density of lymphatic vessels labeled with D2-40 was determined by immunohistochemistry. The relationship between the expression of CD44v6 and ICAM-1 and the degree of differentiation and lymph node metastasis of cervical carcinoma was investigated.Normal cervix, cervical squamous cell carcinoma in situ, cervical cancer tissue LVD gradually increased P0.01.The positive expression rate of CD44v6 and LVD in poorly differentiated cervical carcinoma was higher than that in moderately differentiated cervical carcinoma, and the positive rate of CD44v6 and LVD in cervical carcinoma with lymph node metastasis was higher than that without lymph node metastasis (P0.01).There was a positive correlation between ICAM-1 and LVD in cervical cancer tissue.Conclusion CD44v6 plays an important role in the progression of cervical cancer. It can be used as an effective index to judge lymph node metastasis and diagnosis of cervical cancer, which can be combined with ICAM-1 and LVD to promote the development of cervical cancer.
【作者单位】：江西省妇幼保健院妇科肿瘤科;南昌市第三医院急重症科;
【基金】：江西省卫生计生委科技计划(20155545)
【分类号】：R737.33

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