array CGH和SNP array技术在胚胎植入前遗传学诊断中的应用比较

发布时间：2018-04-04 03:48

本文选题：易位　切入点：遗传　出处：《实用医学杂志》2015年17期

【摘要】：目的:比较两种微阵列技术在不同时期活检及移植时机对染色体易位携带者行胚胎植入前遗传学诊断(PGD)对诊断结果及妊娠结局的影响。方法:对染色体易位携带者行PGD的152个活检周期资料进行回顾性分析,其中60个a CGH-PGD周期采用D3卵裂球活检并新鲜周期移植的策略,92个SNP-PGD周期采用滋养外胚层活检并冷冻周期移植的策略,将两组的诊断结果及妊娠结局进行比较。结果:SNP-PGD与a CGH-PGD在无胚胎移植周期方面无明显差异;在诊断正常率上,SNP-PGD高于a CGH-PGD,两者间差异有统计学意义;移植周期临床妊娠率方面,SNP-PGD高于a CGH-PGD,而早期流产及胎停率则低于后者。结论:采用滋养外胚层活检并冷冻周期移植可能有助于获得较好的妊娠结局。
[Abstract]:Objective: to compare the effects of two microarray techniques on the diagnosis and pregnancy outcome of chromosomal translocation carriers by pre-implantation genetic diagnosis (PGD) at different stages of biopsy and transplantation.Methods: the data of 152 biopsy cycles of chromosomal translocation carriers undergoing PGD were retrospectively analyzed.Sixty a CGH-PGD cycles were performed with D3 cleavage biopsy and fresh cycle transplantation, 92 SNP-PGD cycles with trophoectodermal biopsy and cryopreserved cycle transplantation. The diagnostic results and pregnancy outcomes of the two groups were compared.Results there was no significant difference between CGH-PGD and SNP-PGD in the absence of embryo transfer cycle, the normal rate of SNP-PGD was higher than that of a CGH-PGD, and the clinical pregnancy rate of SNP-PGD was higher than that of a CGH-PGD, while the rate of early abortion and fetal suspension was lower than that of the latter.Conclusion: trophoblast ectodermal biopsy and cryopreservation may contribute to better pregnancy outcome.
【作者单位】：中山大学附属第一医院生殖医学中心广东省生殖医学重点实验室;
【基金】：中山大学青年教师培育计划项目(编号:12ykpy23) 广州市科技计划项目(编号:201300000097)
【分类号】：R714.8

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