环氧化酶-2基因启动子区甲基化水平在子宫内膜异位症中的作用

发布时间：2018-04-04 17:36

  本文选题：子宫内膜异位症　切入点：甲基化　出处：《山东大学学报(医学版)》2017年01期

【摘要】：目的检测环氧化酶-2(COX-2)基因启动子区CRE位点甲基化状况,探讨其在子宫内膜异位症(EMs)发生中的作用。方法收集南昌大学第一附属医院40例子宫内膜异位症患者的子宫内膜作为病例组,同期40例正常子宫内膜作为对照组,采用焦磷酸盐测序技术检测病例组和对照组子宫内膜组织中COX-2基因启动子区CRE位点甲基化频率;采用实时定量PCR及免疫组化技术分别检测病例组和对照组子宫内膜组织中COX-2 mRNA及蛋白的表达;分析COX-2基因启动子区CRE位点甲基化频率与COX-2表达水平的相关性。结果对照组内膜组织COX-2基因启动子区CRE位点的甲基化频率(40.81%±6.10%)显著高于病例组(14.74%±2.84%),P0.05;而对照组COX-2 mRNA表达水平(0.98±0.37)及蛋白表达水平(1.00±0.73)显著低于病例组的mRNA表达水平(2.63±0.44)及蛋白表达水平(2.74±1.18),P均0.05。随着COX-2基因启动子区CRE位点甲基化频率升高,COX-2基因表达水平下降,二者呈负相关(P0.05)。结论 COX-2基因启动子区CRE位点去甲基化与EM s患者在位内膜COX-2高表达相关,在EM s的发生、发展中具有重要作用,提示子宫内膜异位症是一种表观遗传性疾病。
[Abstract]:Objective to detect the methylation of CRE site in the promoter of cyclooxygenase-2 moxigenin (COX-2) gene and to explore its role in the pathogenesis of endometriosis.Methods the endometrium of 40 patients with endometriosis and 40 normal endometrium as control group were collected from the first affiliated Hospital of Nanchang University.The methylation frequency of CRE site in the promoter region of COX-2 gene was detected by pyrophosphate sequencing in the endometrium of the case group and the control group.The expression of COX-2 mRNA and protein in endometrial tissues of case group and control group were detected by real-time quantitative PCR and immunohistochemistry, and the correlation between CRE methylation frequency of COX-2 gene promoter and COX-2 expression was analyzed.缁撴灉瀵圭収缁勫唴鑶滅粍缁嘋OX-2鍩哄洜鍚�鍔ㄥ瓙鍖篊RE浣嶇偣鐨勭敳鍩哄寲棰戠巼(40.81%卤6.10%)鏄捐憲楂樹簬鐥呬緥缁，

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