复发性流产患者蜕膜组织及外周血中TNF-α的表达

发布时间：2018-04-05 07:18

本文选题：复发性流产　切入点：病因　出处：《大连医科大学》2014年硕士论文

【摘要】：目的：比较复发性流产患者外周血和蜕膜组织中TNF-α的表达水平。比较不同流产次数的复发性流产患者TNF-α水平。比较TNF-α对原发性、继发性复发性流产的影响。通过对TNF-α的测定，为临床治疗复发性流产提供理论依据。方法：选取2013.1.1～2014.3.1于大连医科大学附属大连市妇产医院门诊手术室行人工流产术的早期妊娠复发性流产患者76例作为病例组，同期正常妊娠者100例作为对照组。用ELISA方法测定两组患者外周血和蜕膜组织中TNF-α的水平。同时比较不同流产次数的复发性流产患者TNF-α水平，以及TNF-α对原发性、继发性复发性流产的影响。结果：（1）复发性流产组患者的外周血中TNF-α表达水平明显高于正常妊娠组，差异有统计学意义（p=0.000）；复发性流产组患者的蜕膜组织中TNF-α表达水平亦明显高于对照组，差异有显著统计学意义（p=0.000）。（2）2次复发性流产患者外周血中TNF-α表达水平与3次的比较，差异无统计学意义（p=0.200）；与3次以上的比较，差异有统计学意义（p=0.000）；3次与3次以上复发性流产患者TNF-α表达水平比较，，差异有统计学意义（p=0.018）。（3）2次复发性流产患者蜕膜组织中TNF-α表达水平与3次的比较，差异无统计学意义（p=0.256）；与3次以上的比较，差异有统计学意义（p=0.001）；3次与3次以上的比较，差异有统计学意义（p=0.014）。（4）原发性RSA组患者的外周血中TNF-α表达水平明显低于继发性RSA组，差异有显著统计学意义（p=0.000）；继发性RSA组患者的蜕膜组织中TNF-α表达水平明显高于原发性RSA组，差异有统计学意义（p=0.002）。结论：（1）TNF-α在复发性流产患者外周血和蜕膜组织中的表达均异常升高，其可能为导致RSA发生的病因之一。TNF-α在外周血和蜕膜组织中的表达一致，外周血中检测到的TNF-α水平能同时反映局部组织中的水平。（2）RSA发生3次及以上，TNF-α表达水平随着流产次数增加而增高，说明其可能对此次妊娠结局有重要参考价值，可作为临床预测RSA发生的指标。（3）继发性RSA患者外周血和蜕膜组织中的TNF-α表达水平均显著高于原发性RSA患者，说明TNF-α对继发性RSA的发生影响更大。
[Abstract]:Objective: to compare the expression of TNF- 伪 in peripheral blood and decidua of recurrent abortion patients.The levels of TNF- 伪 in recurrent abortion patients with different abortion times were compared.To compare the effect of TNF- 伪 on primary and secondary recurrent abortion.The determination of TNF- 伪 provides a theoretical basis for clinical treatment of recurrent abortion.Methods: 76 cases of early pregnancy recurrent abortion were selected as case group and 100 cases of normal pregnancy as control group, which were performed in the operating room of Dalian Obstetrics and Gynecology Hospital affiliated to Dalian Medical University on January 1, 2014.2014.The patients with recurrent early pregnancy were selected as the case group.The levels of TNF- 伪 in peripheral blood and decidua were measured by ELISA.At the same time, the levels of TNF- 伪 and the effect of TNF- 伪 on primary and secondary recurrent abortion were compared.Results the expression of TNF- 伪 in peripheral blood of recurrent abortion group was significantly higher than that of normal pregnancy group, and the expression of TNF- 伪 in decidual tissue of recurrent abortion group was significantly higher than that of control group.The expression of TNF- 伪 in peripheral blood of patients with recurrent abortion was significantly higher than that of patients with recurrent abortion. There was no significant difference in the expression of TNF- 伪 between the patients with recurrent abortion and three times, but there was no significant difference in the expression of TNF- 伪 between the patients with recurrent abortion and the patients with recurrent abortion more than 3 times.The expression of TNF- 伪 in decidua of patients with recurrent abortion was significantly higher than that in patients with recurrent abortion for 3 times and more than 3 times, and the expression level of TNF- 伪 in decidua of patients with recurrent abortion was significantly higher than that in patients with recurrent abortion for 3 times, and the expression level of TNF- 伪 in decidua of patients with recurrent abortion was significantly higher than that in patients with recurrent abortion for more than 3 times.The expression of TNF- 伪 in decidua in secondary RSA group was significantly higher than that in primary RSA group, and the difference was statistically significant.Conclusion the expression of TNF- 伪 in peripheral blood and decidua of patients with recurrent abortion is abnormally high, which may be one of the causes of RSA. The expression of TNF- 伪 in peripheral blood and decidua is consistent.The level of TNF- 伪 detected in peripheral blood can reflect the level of TNF- 伪 in local tissues at the same time. The expression level of TNF- 伪 increases with the number of miscarriages, which indicates that TNF- 伪 expression may be of important reference value to the outcome of pregnancy.The expression of TNF- 伪 in peripheral blood and decidua in patients with secondary RSA was significantly higher than that in patients with primary RSA, indicating that TNF- 伪 had more influence on the occurrence of secondary RSA.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.21

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