宫内HCMV感染对新生大鼠神经行为发育及远期学习记忆、运动和情绪的影响

发布时间：2018-04-05 10:12

本文选题：人巨细胞病毒　切入点：宫内感染　出处：《安徽医科大学》2014年硕士论文

【摘要】：目的探讨宫内人巨细胞病毒（Human Cytomegalovirus，HCMV）感染对新生大鼠神经行为发育及远期学习记忆、运动和情绪的影响。方法建立宫内HCMV感染SD大鼠模型。随机从实验组及对照组抽取存活的子代大鼠各10只。监测子代大鼠婴儿期神经行为发育，包括生理发育：体重增长、开眼、竖耳；神经发育：听觉惊愕试验、悬崖回避试验、负趋地性试验、前肢握力试验及平面翻正试验；于1月龄、3月龄和6月龄时分别对实验组与对照组进行Morris水迷宫、悬吊实验、旷场实验等神经行为学检测，实验中从两组中随机选取1月龄时、6月龄时子代大鼠，分别采用光镜、电镜观察两组脑组织病理动态变化特点。结果1、与正常对照组比较，实验组新生大鼠生理发育迟缓，表现为体重增长缓慢；开眼、张耳达标率低，差异均有统计学意义(P0.05)；神经发育延迟，与正常对照组比较，表现为听觉惊愕试验、负趋地性试验达标率低；悬崖回避试验、平面翻正试验时间明显延迟；前肢握力试验时间缩短，差异均有统计学意义(P0.05)。2、学习记忆方面：实验组1月龄、3月龄、6月龄时的Morris水迷宫逃避潜伏期、游泳距离均显著高于对照组,同时其穿越平台次数显著低于对照组，且随着年龄增加，其穿越平台能力下降越明显，均有统计学差异(P 0.01)；运动能力方面：实验组大鼠1月龄、3月龄、6月龄时，悬吊实验得分均显著低于对照组，，差异具有统计学意义（P0.001）；情感行为方面：旷场试验结果：实验组大鼠1月龄、3月龄、6月龄时，跨格子数、站立次数均显著低于对照组，差异具有显著性（P0.05）。3、实验组新生大鼠生后1月龄时，海马层轻度损伤、神经髓鞘轻度溶解，6月龄时损伤加重，即海马结构明显紊乱，髓鞘严重变形、溶解显著。结论1、宫内HCMV感染新生大鼠婴儿期神经行为发育较对照组明显迟缓。2、宫内HCMV感染子代大鼠远期空间学习记忆功能明显受损，同时伴有运动和情绪行为发育的异常，且宫内HCMV感染子代大鼠神经行为受损程度与脑组织病理变化一致。
[Abstract]:Objective to investigate the effects of intrauterine human cytomegalovirus (HCMV) infection on neurobehavioral development, long-term learning and memory, motor and emotion in neonatal rats.Methods SD rat model of intrauterine HCMV infection was established.Ten surviving offspring rats were randomly selected from the experimental group and the control group.To monitor the neurobehavioral development in infant rats, including physiological development: weight gain, open eyes, vertical ears, nerve development: auditory consternation test, cliff avoidance test, negative earth-tendency test, forelimb grip strength test and plane-reversal test.Morris water maze, suspension test, open field test and other neurobehavioral tests were performed in the experimental group and the control group at the age of 3 months and 6 months respectively. The offspring rats of 1 month old and 6 month old were randomly selected from the two groups.The pathological changes of brain tissue in two groups were observed by light microscope and electron microscope respectively.Results 1.Compared with the normal control group, the neonatal rats in the experimental group showed slow weight growth, low open eyes, low ear opening rate and significant difference (P 0.05), delayed nerve development, and compared with the normal control group.The results were as follows: auditory consternation test, negative earth-tendency test, low achievement rate, cliff avoidance test, plane inversion test time obviously delayed, forelimb grip force test time shortened,In learning and memory, the escape latency and swimming distance of Morris water maze in the experimental group were significantly higher than those in the control group at the age of 3 months and 6 months, and the times of crossing the platform were significantly lower than those in the control group.With the increase of age, the ability of traversing platform decreased significantly (P 0.01), and the motor ability of the experimental group was significantly lower than that of the control group at the age of 1 month and 3 months and 6 months old, and the scores of the experimental group were significantly lower than those of the control group, and that of the experimental group was significantly lower than that of the control group.The difference was statistically significant (P 0.001); emotional behavior: the results of open field test showed that when the rats in the experimental group were 3 months old and 6 months old, the number of cross grids and the number of standing times were significantly lower than those in the control group.The difference was significant (P 0.05). At the age of 1 month after birth, the hippocampal layer was slightly damaged in the experimental group, and the injury was aggravated at the age of 6 months after the slight dissolution of the myelin sheath, that is, the structure of the hippocampus was obviously disordered, the myelin sheath was seriously deformed and the dissolution was significant.Conclusion 1. The neurobehavioral development of neonatal rats infected with intrauterine HCMV was significantly slower than that of the control group, and the long-term spatial learning and memory function was significantly impaired in the offspring of intrauterine HCMV infection, accompanied by abnormal motor and emotional behavior development.The degree of neurobehavioral impairment in intrauterine HCMV infected offspring was consistent with the pathological changes of brain tissue.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.251

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