uPA在子宫内膜异位症患者在位内膜腺上皮细胞中的表达及其与侵袭性的关系

发布时间：2018-04-05 16:11

本文选题：子宫内膜异位症　切入点：尿激酶型纤溶酶原激活物　出处：《实用医学杂志》2017年03期

【摘要】：目的:探讨尿激酶型纤溶酶原激活物(uPA)在子宫内膜异位症(EMS)患者在位内膜腺上皮细胞中的表达和该因子与其侵袭性的关系。方法:通过原代细胞培养EMS患者和正常内膜各9例在位内膜腺上皮细胞并鉴定,分别经由Western blot和RT-PCR检测该因子的表达。通过侵袭小室验证uPA与腺上皮细胞侵袭性和迁移性的关系。结果:(1)uPA蛋白及mRNA在EMS患者在位内膜腺上皮细胞中的表达比正常内膜中明显增强,其差异具有统计学意义(均P0.05);(2)EMS患者在位内膜腺上皮细胞的侵袭性较正常细胞明显增强,其差异具有统计学意义(均P0.05)。结论 :uPA在EMS患者在位内膜腺上皮细胞中的表达明显增加,且与其侵袭性及迁移能力明显相关,揭示uPA与该病的发生密切相关。
[Abstract]:Aim: to investigate the expression of urokinase-type plasminogen activator (UPA) in eutopic endometrial glandular epithelial cells (EMSs) and its relationship with invasion.Methods: the glandular epithelial cells of 9 patients with EMS and 9 normal endometrium were cultured and identified. The expression of the factor was detected by Western blot and RT-PCR, respectively.The relationship between uPA and invasion and migration of glandular epithelial cells was verified by invasive chamber.The difference was statistically significant (all P 0.05).Conclusion the expression of WUPA in eutopic endometrial glandular epithelial cells of EMS patients is significantly increased, and is significantly related to its invasion and migration ability. It is revealed that uPA is closely related to the occurrence of the disease.
【作者单位】：榆林市星元医院妇产科;西安医学院第一附属医院;榆林市第二医院妇产科;宁夏医科大学总医院妇科;
【基金】：国家自然科学基金(编号:81160078)
【分类号】：R711.71

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