E-Cad在稽留流产患者中的表达及临床意义

发布时间：2018-04-07 18:25

本文选题：稽留流产　切入点：E-Cad　出处：《广西医科大学》2014年硕士论文

【摘要】：目的:研究上皮型钙黏附蛋白（E-Cad）在稽留流产患者血清、绒毛和蜕膜组织的表达，探讨稽留流产的发病机制，为其早期诊断和临床防治提供新的理论基础及实验依据。方法:收集2013年8月至2013年11月我院计划生育科病房收治的稽留流产患者25例（实验组），同期在计生科门诊就诊要求终止妊娠的正常早孕妇女25例（对照组），两组行人工流产术前均抽血，ELISA法检测血清中E-Cad的表达水平；人工流产术后分别收集两组绒毛和蜕膜新鲜组织，采取免疫组织化学方法对两组绒毛及蜕膜组织E-Cad蛋白表达进行组织学定位和定量分析。结果:稽留流产组孕妇血清E-Cad的浓度为（2.96±0.51）ng/mL，显著高于对照组（2.02±0.43）ng/mL，两组比较差异有统计学意义（P0.0001）。在绒毛组织，E-Cad主要表达于滋养细胞胞膜上，稽留流产组绒毛组织E-Cad蛋白表达水平高于正常早孕组，两组比较差异有统计学意义(P＜0.0001)。E-Cad主要表达于蜕膜腺体细胞的胞膜上，蜕膜间质细胞未见阳性表达，稽留流产组蜕膜E-Cad蛋白表达水平高于正常早孕组，两组比较差异有统计学意义(P＜0.0001)。结论:稽留流产患者血清、绒毛和蜕膜组织E-Cad的表达水平均增高，提示稽留流产的发生可能与E-Cad增高有关，机制可能是异常高表达的E-Cad致使滋养细胞的侵袭能力下降，，影响滋养细胞的分化增殖，胎盘着床和血管重铸障碍，导致流产的发生。
[Abstract]:Objective: to study the expression of E-Cadc in serum, villi and decidua of patients with missed abortion, to explore the pathogenesis of missed abortion, and to provide a new theoretical and experimental basis for its early diagnosis, clinical prevention and treatment.Methods: from August 2013 to November 2013, 25 patients with missed abortion in family planning ward of our hospital (experimental group) and 25 normal early pregnant women (control group, control group) who requested termination of pregnancy in the outpatient clinic of family planning department were collected from August 2013 to November 2013.The expression of E-Cad in serum was detected by Elisa before artificial abortion.The expression of E-Cad protein in chorionic villi and decidua tissues of the two groups were detected by immunohistochemical method. The expression of E-Cad protein in the chorionic villi and decidua tissues of the two groups were quantitatively analyzed by immunohistochemical method.Results: the serum E-Cad concentration of pregnant women with missed abortion was 2.96 卤0.51 ng / mL, which was significantly higher than that of control group (2.02 卤0.43 ng / mL). The difference between the two groups was statistically significant (P 0.0001).The expression of E-Cad in chorionic villi was mainly expressed on trophoblast membrane, and the expression of E-Cad protein in chorionic villi of missed abortion group was higher than that in normal early pregnancy group. The difference between the two groups was significant (P < 0.05). The expression of E-Cad was mainly expressed on the membrane of decidua glandular cells.No positive expression was found in decidual stromal cells, and the expression of decidual E-Cad protein in missed abortion group was higher than that in normal early pregnancy group (P < 0.0001).Conclusion: the expression of E-Cad in serum, villi and decidua of patients with missed abortion is increased, suggesting that the occurrence of missed abortion may be related to the increase of E-Cad, and the mechanism may be that the abnormal high expression of E-Cad can decrease the invasion ability of trophoblastic cells.It affects the differentiation and proliferation of trophoblastic cells, placental implantation and vascular remodeling, leading to miscarriage.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.21

【参考文献】