Ezrin及FAK在宫颈鳞癌中的表达及意义

发布时间：2018-04-09 10:39

本文选题：Ezrin　切入点：FAK　出处：《蚌埠医学院》2017年硕士论文

【摘要】：目的:在宫颈鳞癌组织上进行Ezrin及FAK蛋白的检测,并评估两种蛋白在宫颈鳞癌中的表达及意义。方法:在30例正常宫颈(Normal Cervix,NC)、30例低级别鳞状上皮内病变宫颈(Low grade squamous intraepithelial lesions cervix,LSIL)、30例高级别鳞状上皮内病变宫颈(High grade squamous intraepithelial lesions cervix,HSIL)和60例宫颈鳞癌(Squamous carcinoma of the cervix,SCC)组织上,利用QDs标记的链霉亲和素复合物(QDs labeled streptavidin complexes,QDs-SA)能与生物素化二抗Ig G结合的特点分别检测Ezrin和FAK的蛋白表达。利用免疫组化SP法检测宫颈鳞癌组织中Ezrin和FAK的表达水平。结果:1.SP法观察到Ezrin、FAK蛋白分别着色于宫颈细胞的胞膜和胞浆,呈棕黄色,且在宫颈鳞癌细胞中阳性表达率分别为75%、80%,与量子点免疫荧光法(QDs-IHC法)结果相比较,差异均有统计学意义(P0.05);2.QDs-IHC法观察到Ezrin、FAK蛋白分别定位于宫颈细胞的胞膜和胞浆,定位准确,并且阳性信号分别呈现绿色、橙红色的光;3.60例SCC组织中Ezrin蛋白及FAK蛋白阳性表达率分别为96.66%及95.00%,与HSIL组相比差异有统计学意义(P0.05)。30例HSIL组织中Ezrin和FAK蛋白阳性表达率分别为83.33%和80.00%,与LSIL组相比差异有统计学意义(P0.05)。30例LSIL组织中Ezrin和FAK蛋白阳性表达率分别为60.00%和53.33%,与正常宫颈组相比差异无统计学意义(P0.05)。结论:Ezrin及FAK的阳性过表达在宫颈鳞癌的发生发展和侵袭过程中具有一定临床意义,值得深入研究。QDs-IHC法同时检测多个指标优于SP法。
[Abstract]:Objective: to investigate the expression of Ezrin and FAK in cervical squamous cell carcinoma (SCC).The protein expression of Ezrin and FAK were detected by QDs labeled labeled streptavidin complex QDs-SAs and biotinylated second antibody Ig G, respectively.Immunohistochemical SP method was used to detect the expression of Ezrin and FAK in cervical squamous cell carcinoma.Results 1. The Ezrininus FAK protein was stained in the cell membrane and cytoplasm of cervix, and the positive expression rate of Ezrinn FAK protein in cervical squamous cell carcinoma cells was 75 and 80, respectively, compared with the QDs-IHC method by quantum dot immunofluorescence (QDs-IHC).The difference was statistically significant. 2.QDs-IHC method showed that Ezrinus FAK protein was located in the membrane and cytoplasm of cervical cells, and the positive signal was green.The positive expression rates of Ezrin protein and FAK protein were 96.66% and 95.00 in orange-red SCC tissues, respectively. The positive expression rates of Ezrin and FAK protein in HSIL tissues were 83.33% and 80.005%, respectively, which were lower than those in LSIL group.The positive expression rates of Ezrin and FAK protein in 30 cases of LSIL were 60.00% and 53.33, respectively. There was no significant difference compared with normal cervix group (P 0.05).Conclusion the positive overexpression of Ezrin and FAK has a certain clinical significance in the development and invasion of squamous cell carcinoma of cervix, and it is worth further studying that the method of QDs-IHC can detect many indexes at the same time, which is superior to SP method.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.33

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