不同浓度ox-LDL对大鼠原代卵泡膜细胞增殖及激素合成相关基因LXR-α和StAR表达的影响

发布时间：2018-04-09 16:01

本文选题：卵泡膜细胞　切入点：氧化型低密度脂蛋白　出处：《上海交通大学学报(医学版)》2017年03期

【摘要】：目的·初步探讨ox-LDL对大鼠卵泡膜细胞增殖和雄激素合成相关基因LXR-α和St AR表达的影响。方法·免疫组化检测大鼠卵巢组织中LXR-α的表达。体外分离培养原代大鼠卵泡膜细胞,分别用25、50、100、150、200、300和400 mg/L的ox-LDL处理,用real-time PCR检测LXR-αm RNA的变化,用MTT检测细胞活力,用Western blotting检测LXR-α和St AR蛋白的表达。结果·ox-LDL对大鼠卵泡膜细胞增殖的影响和对LXR-α和St AR表达的调控呈现浓度依赖性变化。ox-LDL刺激24 h后,低浓度的ox-LDL(25~150 mg/L)可诱导卵泡膜细胞增殖,以100 mg/L的ox-LDL对卵泡膜细胞增殖的促进作用显著。而当ox-LDL浓度继续上升,细胞存活率下降,以400 mg/L的ox-LDL对卵泡膜细胞增殖的抑制作用显著。低浓度ox-LDL(25~150 mg/L)刺激,导致LXR-αm RNA的表达升高,其中150 mg/L ox-LDL对LXR-αm RNA的表达量影响显著。高浓度ox-LDL抑制LXR-αm RNA的表达,其中400 mg/L的ox-LDL对LXR-αm RNA的表达量影响显著。150 mg/L ox-LDL促进大鼠卵泡膜细胞LXR-α和St AR蛋白的表达上升,但150 mg/L ox-LDL对St AR蛋白表达的促进作用,与对照组相比,差异没有统计学意义;而400 mg/L ox-LDL能显著抑制大鼠卵泡膜细胞LXR-α和St AR蛋白的表达。结论·低浓度的ox-LDL可诱导卵泡膜细胞增殖,促进LXR-α和St AR的表达,而高浓度oxLDL降低细胞活力,抑制LXR-α和St AR的表达。
[Abstract]:Objective to investigate the effects of ox-LDL on proliferation and androgen synthesis related genes LXR- 伪 and St AR expression in rat follicular membrane cells.Methods the expression of LXR- 伪 in rat ovary was detected by immunohistochemistry.Primary rat follicular membrane cells were isolated and cultured in vitro. The cells were treated with 2550100150200300 and 400 mg/L ox-LDL respectively. The changes of LXR- 伪 m RNA were detected by real-time PCR, the cell viability was detected by MTT, and the expression of LXR- 伪 and St AR proteins were detected by Western blotting.Results the effects of ox-LDL on the proliferation of rat follicular membrane cells and the regulation of LXR- 伪 and St AR expression showed concentration-dependent changes. After 24 h of stimulation with ox-LDL, low concentration of ox-LDL(25~150 mg / L could induce the proliferation of follicular membrane cells.The effect of 100 mg/L ox-LDL on the proliferation of follicular membrane cells was significant.However, when the concentration of ox-LDL continued to increase, the cell survival rate decreased, and the inhibitory effect of 400 mg/L ox-LDL on the proliferation of follicular membrane cells was significant.Low concentration of ox-LDL(25~150 mg / L) stimulated the expression of LXR- 伪 m RNA, and 150 mg/L ox-LDL significantly affected the expression of LXR- 伪 m RNA.400 mg/L ox-LDL could significantly inhibit the expression of LXR- 伪 and St AR protein in rat follicular membrane cells.Conclusion low concentration of ox-LDL can induce the proliferation of follicular membrane cells and promote the expression of LXR- 伪 and St AR, while high concentration of oxLDL can decrease cell viability and inhibit the expression of LXR- 伪 and St AR.
【作者单位】：重庆医科大学附属第一医院妇产科;重庆市人口和计划生育科学技术研究院国家卫生计生委出生缺陷与生殖健康重点实验室;
【基金】：重庆市卫生和计划生育委员会2011年医学科研计划项目(2011-2-104)~~
【分类号】：R711.75

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