人卵丘细胞GREM1基因表达与卵母细胞发育潜能关系研究

发布时间：2018-04-10 14:14

本文选题：卵丘细胞 + GREM　；参考：《中国实用妇科与产科杂志》2015年06期

【摘要】：目的探讨辅助生殖技术中人卵丘细胞GREM1基因表达水平与卵母细胞发育潜能的关系。方法回顾性分析福建泉州市妇幼保健生殖医学中心2012年3月至2014年3月接受卵胞浆内单精子显微注射-胚胎移植(ICSIET)治疗的男方因素不孕妇女42例的160个卵母细胞,以形态学评分标准将正常受精的胚胎分为优质胚胎30个和非优质胚胎70个,移植后剩余胚胎冷冻保存或囊胚培养。根据卵母细胞成熟与否、D3胚胎质量及D5/D6囊胚培养情况,实时荧光PT-PCR分别检测其卵丘细胞中GREM1表达水平的差异。结果成熟卵母细胞的卵丘细胞GREM1表达水平比不成熟卵母细胞高(1.9739±0.5114 vs.1.5105±0.2629,P0.001),同样在D3优质胚胎中,检测其卵丘细胞GREM1表达水平比非优质胚胎中的高(2.2693±0.4193 vs.1.9263±0.4912,P0.05);进一步的囊胚培养发现,D5/D6囊胚培养成功的卵丘细胞GREM1表达水平比失败组的明显升高(2.3874±0.2999 vs.1.5943±0.2078,P0.001)。结论卵丘细胞GREM1表达水平与卵母细胞成熟及胚胎质量有关,成熟的卵母细胞与后期形成的胚胎质量密切相关,检测卵丘细胞GREM1表达可预测胚胎发育潜能。
[Abstract]:Objective to investigate the relationship between GREM1 gene expression in human cumulus cells and oocyte developmental potential in assisted reproductive technology.Methods 160 oocytes from 42 infertile women with male factors treated with intracytoplasmic sperm microinjection and embryo transfer in Quanzhou Maternal and Child Health and Reproductive Medicine Center, Quanzhou, Fujian Province, from March 2012 to March 2014, were retrospectively analyzed.Normal fertilized embryos were divided into 30 high quality embryos and 70 non-high quality embryos according to morphological score. The remaining embryos were cryopreserved or blastocyst culture after transfer.According to the embryo quality of oocyte maturation and D5/D6 blastocyst culture, the expression of GREM1 in cumulus cells was detected by real-time fluorescence PT-PCR.Results the expression level of GREM1 in cumulus cells of mature oocytes was 1.9739 卤0.5114 vs.1.5105 卤0.2629 vs.1.5105 卤0.2629P0.001, which was higher than that of immature oocytes.The expression level of GREM1 in cumulus cells was 2.2693 卤0.4193 卤0.4912 vs.1.9263 卤0.4912 P0.05G higher than that in non-high quality embryos, and the level of GREM1 expression in successfully cultured cumulus cells of D _ 5 / D _ 6 blastocysts was 2.3874 卤0.2999 vs.1.5943 卤0.2078 P 0.001 higher than that of failed blastocysts.Conclusion the expression level of GREM1 in cumulus cells is related to the maturation of oocytes and the quality of embryos. The expression of GREM1 in cumulus cells is closely related to the quality of embryos formed in the later stage. The detection of GREM1 expression in cumulus cells can predict the developmental potential of embryos.
【作者单位】：福建医科大学泉州市儿童医院妇幼保健院生殖医学中心;福建医科大学附属第二医院检验科;
【基金】：泉州市卫生局2012年卫生科研项目资助福建省卫生厅青年科研课题(2013-2-129) 泉州市科技项目B类课题(Z[2014]0010)
【分类号】：R714.8

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