硫酸镁对子痫前期大鼠模型血清及脑内炎症因子、核因子-κB p65表达的影响

发布时间：2018-04-11 22:16

本文选题：子痫前期 + 炎症因子　；参考：《实用医学杂志》2017年09期

【摘要】：目的:观察硫酸镁对子痫前期大鼠血清及脑内炎症因子、脑内核因子-κB p65(NF-κB p65)表达的影响。方法:健康大鼠随机分4组:正常妊娠组(10只)、硫酸镁对照组(10只)、子痫前期(PE)组(10只)和硫酸镁干预组(10只)。检测大鼠血压、尿蛋白、血尿素氮、丙氨酸氨基转移酶水平;RT-q PCR及ELISA检测大鼠脑内及血清白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)及白细胞介素-6(IL-6)表达;Western blot检测大鼠脑内NF-κB p65表达。结果:第20天PE组收缩压、尿蛋白及血尿素氮显著高于正常妊娠组和硫酸镁干预组(P0.05)。RT-q PCR及ELISA结果显示硫酸镁干预组血清及脑内IL-1β、TNF-α及IL-6含量明显低于PE组(P0.05),高于正常妊娠组(P0.05)。灰度分析示硫酸镁干预组大鼠脑内NF-κB p65蛋白水平明显低于PE组,高于正常妊娠组。结论:硫酸镁可能通过NF-κB p65路径降低子痫前期模型大鼠炎症因子水平防治子痫前期。
[Abstract]:Aim: to observe the effect of magnesium sulfate on the expression of inflammatory factors in serum and brain and NF- 魏 B p65 in preeclampsia rats.Methods: healthy rats were randomly divided into 4 groups: normal pregnancy group (n = 10), magnesium sulfate control group (n = 10), preeclampsia group (n = 10) and magnesium sulfate intervention group (n = 10).Results: on the 20th day, systolic blood pressure, urine protein and blood urea nitrogen in PE group were significantly higher than those in normal pregnancy group and magnesium sulfate intervention group. The results showed that the levels of IL-1 尾 TNF- 伪 and IL-6 in serum and brain of magnesium sulfate intervention group were significantly lower than those in PE group, and higher than that in normal pregnancy group.The level of NF- 魏 B p65 protein in the brain of rats in magnesium sulfate intervention group was significantly lower than that in PE group and higher than that in normal pregnancy group.Conclusion: magnesium sulfate may reduce the level of inflammatory cytokines in preeclampsia rats by NF- 魏 B p65 pathway to prevent and treat preeclampsia.
【作者单位】：湖北医药学院附属襄阳医院襄阳市第一人民医院妇产科;湖北医药学院附属襄阳医院襄阳市第一人民医院病理科;
【基金】：湖北省教育厅科学技术研究项目(编号:B2016135)
【分类号】：R-332;R714.244

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