多囊卵巢综合症患者结合珠蛋白血清水平及基因型的研究

发布时间：2018-04-12 06:49

本文选题：多囊卵巢综合症 + 结合珠蛋白　；参考：《重庆医科大学》2017年硕士论文

【摘要】：目的:探讨多囊卵巢综合症(PCOS)患者血清结合珠蛋白(Hp)水平及其与各代谢指标的相关性;并进一步探讨PCOS患者Hp基因型和基因频率的分布及其与PCOS之间的关系。第一部分:研究对象:PCOS组选择符合PCOS诊断标准的PCOS患者106例,对照组选择无高雄激素血症、月经规律的不孕症妇女86例为研究对象。方法:测定身高、体重,计算体重指数(BMI),根据亚洲人群中正常体重指数指标,将PCOS组分为非肥胖PCOS组(57例)和肥胖PCOS组(49例);行口服葡萄糖耐量试验和胰岛素释放试验,计算稳态模型胰岛素抵抗指数(HOMA-IR),按照胰岛素抵抗(IR)诊断标准,将PCOS组分为胰岛素抵抗PCOS组(45例)和非胰岛素抵抗PCOS组(61例)。酶联免疫吸附法(ELISA)检测所有研究对象血清Hp水平。结果:(1)PCOS组血清Hp水平显著高于对照组(P0.01),(2)肥胖PCOS组血清Hp水平高于非肥胖PCOS组(P0.01);(3)胰岛素抵抗PCOS组血清Hp水平显著高于非胰岛素抵抗PCOS组(P0.01)。(4)血清Hp水平变化与BMI(P0.01)、HOMA-IR(P0.01)正相关结论:PCOS患者血清Hp水平与BMI、HOMA-IR正相关,提示Hp可能与PCOS患者的肥胖和胰岛素抵抗关系密切。结合珠蛋白可能是PCOS诊治过程中的有效观察指标。第二部分:研究对象:PCOS组选择符合PCOS诊断标准的PCOS患者106例,对照组选择无高雄激素血症、月经规律的不孕症妇女86例为研究对象。方法:PCR法检测Hp基因型和基因频率的分布。结果:PCOS组与对照组Hp2-2基因型频率为0.368和0.186;Hp2-1基因型频率为0.424和0.477;Hp1-1基因型频率为0.208和0.337。PCOS组Hp2-2基因型频率显著高于对照组(0.368vs0.186,P0.01),Hp2等位基因频率也高于对照组(0.580vs0.424,P0.01)。结论:Hp2基因在PCOS中有聚集现象,携有Hp2-2基因型的人群可能对PCOS有较高的遗传易感性。
[Abstract]:Objective: to investigate the relationship between serum globin (Hp) level and metabolic indexes in patients with polycystic ovary syndrome (PCOS), and to explore the distribution of HP genotype and gene frequency in patients with PCOS and their relationship with PCOS.Part one: the subjects of the study were 106 PCOS patients who met the diagnostic criteria of PCOS and 86 infertile women who had no hyperandrogenemia and regular menstruation in the control group.Methods: height, body weight, body mass index (BMI) were measured. According to the normal body mass index (BMI) in Asian population, PCOS was divided into non-obese PCOS group (n = 57) and obese PCOS group (n = 49). Oral glucose tolerance test (OGTT) and insulin release test were performed.According to the diagnostic criteria of insulin resistance, PCOS was divided into insulin resistance (PCOS) group (n = 45) and non-insulin resistance (PCOS) group (n = 61).Serum HP levels in all subjects were detected by enzyme linked immunosorbent assay (Elisa).Conclusion Serum HP level in patients with PCOS is positively correlated with BMIOHOMA-IR.The results suggest that HP may be associated with obesity and insulin resistance in PCOS patients.Binding globin may be an effective index in the diagnosis and treatment of PCOS.Part two: one hundred and six PCOS patients who met the diagnostic criteria of PCOS were selected in the study group. 86 infertile women without hyperandrogenemia and regular menstruation were selected as the subjects in the control group.Methods HP genotypes and gene frequencies were detected by PCR.Results the frequencies of Hp2-2 genotypes 0.368 and 0.186 Hp2-1 were 0.424 and 0.477Hp1-1, and the frequencies of Hp2-2 genotype in 0.337.PCOS group were significantly higher than those in control group (0.368vs0.186 vs 0.186 P0.01P0.01a). The frequencies of Hp2 allele were also higher than those of control group (0.580vs0.424P0.01a).Conclusion there is a clustering phenomenon in PCOS with the gene of 1: Hp2, and the population with Hp2-2 genotype may have a higher genetic susceptibility to PCOS.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R711.75

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