miR-101对上皮性卵巢癌SKOV3细胞抗失巢凋亡的作用研究

发布时间：2018-04-13 04:00

本文选题：miR- + 上皮性卵巢癌　；参考：《现代妇产科进展》2017年02期

【摘要】：目的:探讨miRNA-101(miR-101)对上皮性卵巢癌SKOV3细胞抗失巢凋亡的作用,以及在失巢凋亡过程中可能的作用机制。方法:构建失巢凋亡模型模拟卵巢癌细胞转移的生长状态;用脂质体Lipofectamine 2000将miR-101 mimics及miR-NC转染SKOV3细胞,随机分为阴性对照组、miR-空白质粒组(miR-NC组)和miR-101质粒组。RTPCR法检测细胞内miR-101 mRNA表达水平;流式细胞术检测细胞凋亡率;Transwell侵袭实验及划痕实验检测细胞的侵袭及迁移能力;RT-PCR及Western blot法检测c-fos基因mRNA和蛋白表达水平。结果:失巢凋亡模型构建成功,模型中SKOV3细胞呈悬浮、团簇状生长;将miR-101 mimics转染SKOV3细胞后,SKOV3细胞中miR-101表达水平增加,同时癌细胞失巢凋亡增加;透膜细胞数量及细胞迁移距离减少;c-fos mRNA和蛋白表达水平均降低。结论:miR-101过表达可增加SKOV3细胞失巢凋亡,降低细胞侵袭及迁移能力,下调c-fos基因表达可能是其作用分子机制。
[Abstract]:Aim: to investigate the effect of miRNA-101 miR-101) on apoptosis of epithelial ovarian cancer SKOV3 cells and its possible mechanism.Methods: a model of apoptosis without nest was constructed to mimic the growth of ovarian cancer cells, and miR-101 mimics and miR-NC were transfected into SKOV3 cells by liposome Lipofectamine 2000.MiR-101 plasmid group and miR-101 plasmid group were used to detect the expression of miR-101 mRNA in the cells by reverse transcription-polymerase chain reaction (RT-PCR).The rate of apoptosis was detected by flow cytometry and the expression of c-fos gene mRNA and protein was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot assay respectively.Results: the model of apoptosis was successfully constructed, the SKOV3 cells in the model were suspended and clustered, the expression of miR-101 in SKOV3 cells increased after transfection of miR-101 mimics into SKOV3 cells, and the apoptosis of cancer cells increased.The expression of c-fos mRNA and protein decreased in the number of permeable cells and the distance of cell migration.Conclusion the overexpression of miR-101 can increase the apoptosis of SKOV3 cells and decrease the ability of cell invasion and migration. The down-regulation of c-fos gene expression may be the molecular mechanism.
【作者单位】：华北理工大学附属医院妇产科;华北理工大学公共卫生学院;华北理工大学临床学院;
【基金】：河北省临床医学优秀人才培养(No:310103020704)
【分类号】：R737.31

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