人工干预对反复种植失败患者着床窗口期子宫内膜组织中LPAR3和HOXA-11表达的影响

发布时间：2018-04-13 11:03

本文选题：子宫内膜容受性 + 子宫内膜搔刮术　；参考：《郑州大学学报(医学版)》2015年06期

【摘要】：目的:探讨对反复种植失败(RIF)患者进行人工干预后,着床窗口期子宫内膜组织中溶血磷脂酸受体3(LPAR3)和同源框基因11(HOXA-11)表达变化对临床结局的影响。方法:将92例因输卵管因素不孕的RIF患者分为搔刮组(子宫内膜搔刮,n=31)、中成药组(调经促孕丸,n=31)、未干预组(n=30)。采用RT-PCR和Western blot方法检测3组患者窗口期子宫内膜组织中LPAR3和HOXA-11 mRNA和蛋白表达的情况,并观察3组患者临床妊娠率的差异。结果:搔刮组、中成药组患者窗口期子宫内膜组织中LPAR3和HOXA-11 mRNA及蛋白表达量高于未干预组,差异有统计学意义(F=57.463和60.771,P0.001;F=48.816和40.313,P0.001),而前两组比较差异无统计学意义(P0.05)。搔刮组、中成药组复苏周期临床妊娠率分别是48.4%和32.3%,均高于未干预组(16.7%),差异有统计学意义(χ2=6.908,P=0.032),但前两组比较差异无统计学意义(P0.05)。结论:子宫内膜搔刮术和口服调经促孕丸2种干预措施均可以改善子宫内膜容受性,提高RIF患者的临床妊娠率。
[Abstract]:Objective: to discuss the effects of repeated implantation failure (RIF) patients with artificial intervention, lysophosphatidic acid receptors endometrium during the implantation window in 3 (LPAR3) and homeobox gene 11 (HOXA-11) expression affects clinical outcome. Methods: 92 patients with tubal infertility were divided into group RIF scraping (endometrial curettage, n=31), Chinese medicine group (Tiaojing cuyun pill, n=31), non intervention group (n=30). The expression of LPAR3 and HOXA-11 mRNA and protein by RT-PCR and Western blot for the detection of 3 groups of patients with window in endometrial tissue, and observe the difference between the 3 groups of patients with clinical pregnancy rate. Results: the curettage group was higher than that of untreated group LPAR3 and HOXA-11 mRNA and protein expression in endometrial tissue of patients in medicine group in the period of time, the difference was statistically significant (F=57.463 and 60.771, F=48.816 and P0.001; 40.313, P0.001), and no difference between the two groups was statistically significant Meaning (P0.05). Curettage group, Chinese medicine group recovery cycle clinical pregnancy rate were 48.4% and 32.3%, were higher than those of the untreated group (16.7%), the difference was statistically significant (2=6.908, P=0.032), but the difference between the two groups was not statistically significant (P0.05). Conclusion: endometrial curettage and oral Tiaojing cuyun pill 2 interventions can improve endometrial receptivity, improve the clinical pregnancy rate of patients with RIF.

【作者单位】：华中科技大学附属同济医院生殖中心;郑州大学第三附属医院生殖中心;
【基金】：河南省科技攻关计划项目2011020054
【分类号】：R714.8

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