全反式维A酸联合佛波酯引起人子宫内膜基质细胞迅速分泌血管内皮生长因子

发布时间：2018-04-13 22:07

本文选题：全反式维A酸 + 人子宫内膜基质细胞　；参考：《中国妇产科临床杂志》2017年01期

【摘要】：目的检测全反式维A酸(all-trans-retinoic acid,ATRA)和佛波酯(PMA)对人子宫内膜基质细胞(HESC)分泌血管内皮生长因子(VEGF)的增强作用。方法通过ELISA、real time PCR检测ATRA、PMA对在VEGF m RNA和蛋白水平上的影响;通过Western-blotting检测ATRA、PMA对HESC表达VEGF及其受体的影响;观察ATRA对VEGF分泌的剂量和时间依赖性;评价ATRA对VEGF的特异性;使用H_2O_2代替ATRA联合PMA刺激HESC,探讨ATRA介导VEGF分泌增加的可能性途径。结果单独使用ATRA不能改变VEGF的mRNA和蛋白表达,单独使用PMA虽然能够增加VEGF的m RNA表达但并不能增加VEGF蛋白的表达,当共同使用ATRA和PMA时却明显地提高VEGF蛋白的表达量。ATRA对VEGF分泌的促进作用具有明显的剂量和时间依赖性,且该作用是特异性。H2O2可以模拟ATRA增加PMA对HESC分泌VEGF的刺激作用。结论ATRA可以联合PMA迅速增强HESC的VEGF表达,这种作用可能是通过氧化还原反应介导的。
[Abstract]:Objective to investigate the effects of all-trans-retinoic acid ATRAand PMA on the secretion of vascular endothelial growth factor (VEGF) in human endometrial stromal cells (HESCs).Methods the effect of Western-blotting on the expression of VEGF and its receptor in HESC was detected by Western-blotting, the dose and time dependence of ATRA on VEGF secretion was observed, and the specificity of ATRA to VEGF was evaluated.Using H_2O_2 instead of ATRA combined with PMA to stimulate HESCs, to explore the possible pathway of ATRA mediated VEGF secretion increase.Results ATRA alone could not change the expression of mRNA and protein in VEGF. PMA alone could increase the expression of m RNA of VEGF but not the expression of VEGF protein.When ATRA and PMA were used together, the expression of VEGF protein was significantly increased. ATRA enhanced the secretion of VEGF in a dose-and time-dependent manner, and the effect was specific. H2O2 could mimic the stimulation of PMA on HESC VEGF secretion by ATRA.Conclusion ATRA combined with PMA can rapidly enhance the VEGF expression of HESC, which may be mediated by redox reaction.
【作者单位】：内蒙古妇幼保健院妇产科;内蒙古医科大学基础医学院;

【相似文献】