microRNA-320d通过PBX3抑制子宫内膜癌JEC细胞上皮-间质转化功能

发布时间：2018-04-14 16:21

本文选题：子宫内膜癌 + 微小RNA-d　；参考：《中山大学学报(医学科学版)》2017年05期

【摘要】：【目的】研究microRNA-320d(miR-320d)对子宫内膜癌JEC细胞上皮-间质转化功能的影响及其相关机制。【方法】JEC子宫内膜癌细胞株分别转染miR-320d mimics和negative control mimic,分别作为M320d、NCM组,并设立未转染对照control组,采用RT-PCR法检测各组细胞miR-320d含量。Transwell实验检测3组细胞迁移能力和侵袭能力。Westernblot法检测3组细胞α-Catenin和PBX3表达水平,及PBX3过表达对miR-320d抑制EMT的拮抗作用。双荧光素酶实验检测miR-320d与PBX3的关系。【结果】M320d组miR-320d的表达水平为control组的(808.25±15.58)倍(P0.05)。M320d组迁移细胞数量29.56±0.59低于control组的94.48±1.02(P0.05)。M320d组侵袭细胞数量7.33±0.84低于control组的86.28±3.51(P0.05)。M320d组细胞α-Catenin、E-cadherin蛋白表达量(0.365±0.017、0.261±0.008)高于对照组(0.114±0.010、0.151±0.021),Vimentin蛋白表达量(0.105±0.009)低于对照组(0.211±0.025),PBX3蛋白表达量(0.181±0.002)低于对照组(0.311±0.007)。PBX3过表达M320d组子宫内膜癌细胞中α-Catenin、E-cadherin蛋白表达量(0.139±0.019、0.143±0.007)低于对照组(0.399±0.034、0.261±0.017),Vimentin蛋白表达量(0.234±0.008)高于对照组(0.105±0.009),差异均有统计学意义(P0.05)。双荧光素酶检验结果显示PBX3为miR-320d的下游靶基因。【结论】miR-320d可能通过降低下游靶基因PBX3水平影响EMT相关蛋白表达,抑制子宫内膜癌JEC细胞的上皮-间质转化功能。
[Abstract]:[objective] to study the effect of microRNA-320dnmiR-320d on the epithelial-interstitial transformation of endometrial carcinoma JEC cells and its related mechanisms. [methods] JEC endometrial cancer cell lines were transfected into miR-320d mimics and negative control mimic. respectively as M320dNCM group, and untransfected control control group was established.The expression of 伪 -Catenin and PBX3 in the three groups was detected by RT-PCR assay, and the expression of 伪 -Catenin and PBX3 was detected by Western blot, and the antagonistic effect of PBX3 overexpression on the inhibition of EMT by miR-320d.Double luciferase assay was used to detect the relationship between miR-320d and PBX3. [results] the expression level of miR-320d in M320d group was 808.25 卤15.58 in control group. The number of migration cells in M320d group was 29.56 卤0.59 lower than that in control group (94.48 卤1.02(P0.05).M320d group, 7.33 卤0.84), and that in control group (86.28 卤3.51(P0.05).M320d group, 86.28 卤3.51(P0.05).M320d group), the expression of 伪 -Cateninine E-cadherin protein table was lower than that in control group (29.56 卤0.59).The difference was statistically significant (P 0.05).The results of double luciferase assay showed that PBX3 was the downstream target gene of miR-320d. [conclusion] miR-320d may affect the expression of EMT related protein by decreasing the PBX3 level of downstream target gene and inhibit the epithelial-interstitial transformation function of JEC cells in endometrial carcinoma.
【作者单位】：中山大学附属第一医院妇产科;
【基金】：国家自然科学青年基金(81602723) 广东省科技发展专项(公益研究与能力建设,2016A020215214) 广东省医学科研基金(A2015130)
【分类号】：R737.33

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