SYK和BNIP3在子宫内膜癌中的表达及临床意义

发布时间：2018-04-14 19:51

本文选题：SYK + BNIP3　；参考：《广西医科大学》2014年硕士论文

【摘要】：目的：探究脾酪氨酸激酶(spleen tyrosine kinase, Syk)及促凋亡线粒体蛋白（Bcl-2adenocarcinoma E1B19kD interacting protein3，BNip3）在子宫内膜癌组织、子宫内膜不典型增生组织及其正常子宫内膜组织中的表达及其相关临床意义。方法：采用实时定量荧光PCR和免疫组化的方法测定SYK和BNIP3mRNA及其相关蛋白在52例子宫内膜癌组织、14例子宫内膜不典型增生组织及36例正常子宫内膜组织中的表达情况，，分析子宫内膜癌组织中二者的表达水平与临床病理学特征的关系，并对SYK与BNIP3在子宫内膜癌中的表达情况进行相关性检验。结果：1.在正常子宫内膜组、不典型增生组、子宫内膜癌组中SYK的表达呈现一种明显的递减趋势，其mRNA的相对表达量分别为1.44±0.36、0.98±0.23、0.63±0.20，SYK蛋白在正常子宫内膜组的阳性表达率（91.7%）明显高于子宫内膜癌组（32.7%）、不典型增生组（71.4%），差异均有统计学意义（P0.01）； 2. BNIP3mRAN在正常子宫内膜组、不典型增生组、子宫内膜癌组中的相对表达量分别为0.82±0.23、1.99±0.59、1.64±0.46，蛋白水平在子宫内膜不典型增生组（92.9%）的表达明显高于正常子宫内膜组（8.3%）与子宫内膜癌组(86.5%)，三组差异均有统计学意义（P0.01）； 3. SYK与子宫内膜癌的组织分级、临床分期、淋巴结转移、肌层浸润深度有关(P0.05)，而BNIP3mRNA及蛋白的表达水平与子宫内膜癌病人的临床病理特征无显著相关性（P0.05）； 4.子宫内膜癌中SYK与BNIP3两者的表达呈负相关。结论：SYK与BNIP3的异常表达与子宫内膜正常细胞的恶变、浸润、转移等均存在一定的相关性，联合检测SYK及BNIP3的表达情况可能有助于子宫内膜恶性变的早期诊断及预后的判断。
[Abstract]:Objective: to investigate the expression and clinical significance of spleen tyrosine kinase (Spleen tyrosine kinase) and apoptotic mitochondrial protein Bcl-2adenocarcinoma E1B19kD interacting protein 3 (BNip3) in endometrial carcinoma, endometrial atypical hyperplasia and normal endometrium.Methods: the expression of SYK, BNIP3mRNA and their related proteins in 52 cases of endometrial carcinoma tissues, 14 cases of endometrial atypical hyperplasia and 36 cases of normal endometrial tissues were detected by real-time quantitative PCR and immunohistochemistry.To analyze the relationship between the expression level of SYK and BNIP3 and the clinicopathological characteristics of endometrial carcinoma, and to test the correlation between the expression of SYK and BNIP3 in endometrial carcinoma.The result is 1: 1.The expression of SYK in normal endometrium, atypical hyperplasia and endometrial carcinoma showed a decreasing trend.The relative expression of mRNA was 1.44 卤0.36 ~ 0.98 卤0.23 ~ 0.63 卤0.20 ~ 0.20 in normal endometrium, respectively. The positive expression rate of mRNA in normal endometrium was significantly higher than that in endometrial carcinoma (32.7%), and in atypical hyperplasia (71.4%), the difference was statistically significant (P0.01).2.BNIP3mRAN in normal endometrium group, atypical hyperplasia group,The relative expression of protein in endometrial carcinoma group was 0.82 卤0.231.99 卤0.59 卤1.64 卤0.46, respectively. The expression of protein in endometrial atypical hyperplasia group was significantly higher than that in normal endometrial carcinoma group (8.3cm) and endometrial carcinoma group (86.5%). There was significant difference among the three groups (P 0.01).3.SYK was related to histological grade, clinical stage, lymph node metastasis and depth of myometrium invasion of endometrial carcinoma, while the expression of BNIP3mRNA and protein had no significant correlation with the clinicopathological features of endometrial carcinoma.4.The expression of SYK and BNIP3 were negatively correlated in endometrial carcinoma.Conclusion the abnormal expression of SYK and BNIP3 may be related to the malignant change, invasion and metastasis of endometrial normal cells. The combined detection of SYK and BNIP3 may be helpful to the early diagnosis and prognosis of endometrial malignancy.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

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