宫颈癌患者血清骨桥蛋白、可溶性E-钙黏蛋白含量与肿瘤增殖、侵袭活性的相关性

发布时间：2018-04-14 20:09

本文选题：宫颈癌 + 骨桥蛋白　；参考：《中国老年学杂志》2017年18期

【摘要】：目的探讨宫颈癌患者血清骨桥蛋白(OPN)、可溶性E-钙黏蛋白(sE-cadherin)含量与肿瘤增殖、侵袭活性的相关性。方法宫颈癌患者70例为宫颈癌组,宫颈不典型增生患者58例为不典型增生组,宫颈炎症患者60例作为炎症组。入院后当天,采用二抗间接法测定血清OPN含量,采用酶联免疫吸附法(ELISA)测定sE-cadherin含量;采用RT-PCR法测定宫颈组织增殖基因、侵袭基因mRNA表达量。结果宫颈癌组血清OPN含量高于不典型增生组、炎症组,sE-cadherin含量低于不典型增生组、炎症组(P0.05);宫颈癌组的组织增殖基因GBP1、caveolin-1 mRNA表达量低于不典型增生组、炎症组,Bcl-xL、Sp2、EZH2 mRNA表达量高于不典型增生组、炎症组(P0.05);宫颈癌组的组织侵袭基因Prdx4、Sox2、Rab11、RbAp48 mRNA表达量高于不典型增生组、炎症组(P0.05)。宫颈癌血清OPN、sE-cadherin含量与以上肿瘤增殖、侵袭活性基因均有一定的相关性(P0.05)。结论宫颈癌患者血清中OPN高表达、sE-cadherin低表达,其含量与肿瘤增殖、侵袭程度存在直接相关性。
[Abstract]:Objective to investigate the correlation between serum osteopontin (OPN), soluble E-cadherin (E-cadherin) and tumor proliferation and invasion activity in patients with cervical cancer.Methods 70 patients with cervical cancer, 58 patients with atypical hyperplasia of cervix and 60 patients with cervix inflammation were treated as inflammation group.On the day of admission, the content of serum OPN was determined by the second antibody indirect method, the content of sE-cadherin was determined by Elisa, and the expression of proliferative gene and invasive gene mRNA in cervical tissue was determined by RT-PCR method.Results the level of serum OPN in cervical cancer group was higher than that in atypical hyperplasia group, the content of sE-cadherin in inflammatory group was lower than that in atypical hyperplasia group, and the expression of tissue proliferative gene GBP1caveolin-1 mRNA in cervical cancer group was lower than that in atypical hyperplasia group.The expression of EZH2 mRNA in inflammatory group was higher than that in atypical hyperplasia group (P 0.05), and the expression of invasive gene Prdx4, Sox2, Rab11RbAp48 mRNA in cervical carcinoma group was higher than that in atypical hyperplasia group, and the expression level of P0.05 in inflammatory group was higher than that in atypical hyperplasia group.The level of serum OPNNs E-cadherin in cervical cancer was correlated with the proliferation and invasion activity of the tumor (P 0.05).Conclusion the high expression of OPN and the low expression of sE-cadherin in serum of patients with cervical cancer have direct correlation with tumor proliferation and invasion.
【作者单位】：海口市妇幼保健院妇产科;
【分类号】：R737.33

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