维生素E及叶酸对高血糖孕鼠子代心肌细胞凋亡的影响

发布时间：2018-04-15 01:00

本文选题：维生素E + 妊娠糖尿病　；参考：《现代预防医学》2015年02期

【摘要】：目的探讨维生素E(Ve)及叶酸对高血糖诱导的乳鼠心肌细胞凋亡蛋白caspase-3表达的影响。方法将50只清洁级成年SD孕鼠随机分为5组,分别为正常对照组(A组)、妊娠糖尿病组(B组)、叶酸组(C组)、维生素E组(D组)和Ve+叶酸组(E组)。除对照组外,其余大鼠于妊娠d 1腹腔注射链脲佐菌素(STZ)35 mg/kg制备妊娠糖尿病动物模型,对照组给予等量0.1 mmol/L(p H 4.5)柠檬酸钠—柠檬酸缓冲液。孕d 5,D、E 2组予50 mg/kg维生素E灌胃,C、E组予0.4 mg/kg叶酸灌胃,A、B组予等量的生理盐水灌胃,持续7 d。取自然分娩的乳鼠心肌组织于光镜下观察心肌结构的改变,应用免疫组织化学法检测各组乳鼠心肌细胞caspase-3抗原的表达。蛋白免疫印迹(western-blot)检测各组乳鼠心肌细胞caspase-3的表达。结果 (1)造模前各组血糖值比较差异无统计学意义(P0.05);造模后,B、C、D、E组血糖值明显高于A组,差异有统计学意义(P0.01);B、C、D、E 4组间比较差异无统计学意义(P0.05)。(2)光镜下观察B、C、D、E组乳鼠心肌组织呈现不同程度损伤,表现为细胞坏死、水肿、轮廓不清,心肌纤维排列疏松紊乱、炎性细胞浸润。(3)5组乳鼠心肌细胞凋亡蛋白caspase-3的表达不同,差异有统计学意义(P0.01)。结论妊娠糖尿病孕鼠对子代心肌细胞凋亡影响明显,孕期补充维生素E、叶酸对乳鼠心肌细胞凋亡有明显改善作用,且叶酸抑制凋亡的作用优于维生素E,同时补充维生素E和叶酸的效果优于单独补充一种。
[Abstract]:Objective to investigate the effects of Vitamin E (Veg) and folic acid (folic acid) on the expression of apoptotic protein (caspase-3) in neonatal rat cardiomyocytes induced by hyperglycemia.Methods Fifty adult SD pregnant rats of clean grade were randomly divided into 5 groups: normal control group (group A), gestational diabetes group (group B), folic acid group (group C), vitamin E group (group D) and verfolic acid group (group E).In addition to the control group, the other rats were injected intraperitoneally with streptozotocin (STZ) 35 mg/kg on the first day of pregnancy to establish gestational diabetic animal model. The control group was given the same amount of 0.1 mmol/L(p H 4.5) citrate sodium citric acid buffer solution.On the day of gestation, the 50 mg/kg vitamin E group was given 50 mg/kg vitamin E intragastric perfusion, and the 0. 4 mg/kg folic acid intragastric perfusion group was given the same amount of normal saline for 7 days.The changes of myocardial structure were observed under light microscope and the expression of caspase-3 antigen in neonatal rat cardiomyocytes was detected by immunohistochemical method.Western blotting was used to detect the expression of caspase-3 in neonatal rat cardiomyocytes.Results (1) there was no significant difference in blood glucose levels between the three groups before model making (P 0.05), but the blood glucose level in group B (C) was significantly higher than that in group A (P < 0.05), and the blood glucose level was significantly higher than that in group A (P < 0.05).There was no significant difference between the two groups (P0.05) under light microscope, the myocardial tissue of the neonatal rats in the BCU DU E group showed different degrees of injury, such as cell necrosis, edema, unclear outline and loose arrangement of myocardial fibers.The expression of apoptotic protein caspase-3 in neonatal rat cardiomyocytes was different in inflammatory cell infiltration group (P 0.01).Conclusion pregnant rats with gestational diabetes mellitus have obvious effects on the apoptosis of cardiac myocytes in their offspring. Vitamin E and folic acid supplementation during pregnancy can obviously improve the apoptosis of cardiac myocytes in neonatal rats.The inhibitory effect of folic acid on apoptosis was superior to that of vitamin E and vitamin E and folic acid supplementation.
【作者单位】：浙江萧山医院心内科;广西医科大学;揭阳市人民医院心内科;
【基金】：广西自然科学基金重点项目(2010GXNSFD013054)
【分类号】：R714.256

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