临产前后子宫平滑肌组织中G蛋白偶联受体30的表达及其临床意义研究

发布时间：2018-04-20 22:09

本文选题：GPR + 子宫平滑肌　；参考：《中国实用妇科与产科杂志》2015年07期

【摘要】：目的探讨G蛋白偶联受体30(GPR30)m RNA和蛋白在临产前、后子宫平滑肌组织中的表达及其与分娩发动的相关性。方法 2011年3月至2012年3月在青岛市市立医院选取临产前进行选择性剖宫产的足月妊娠妇女作为未临产组(n=40),自然临产进入产程活跃期后进行急症剖宫产的足月妊娠妇女作为临产组(n=50),于剖宫产术中取少量子宫平滑肌组织,采用实时荧光定量PCR技术和Western blot技术检测两组子宫平滑肌组织中GPR30 m RNA和蛋白的表达量;并于术前取静脉血,采用放射免疫法测定血清中雌二醇(E2)、雌三醇(E3)以及孕酮(P)的含量。结果实时荧光定量PCR方法检测临产组和未临产组子宫平滑肌组织中均有GPR30 m RNA表达,临产组样本△Ct为3.08±0.35,未临产组样本△Ct为4.02±0.67,差异具有统计学意义(P0.05),用双△Ct法进行相对定量分析,临产组GPR30 m RNA表达量是未临产组的1.92倍。Western blot技术检测临产组和未临产组子宫平滑肌组织中均有GPR30蛋白表达,临产组GPR30蛋白相对表达量4.06±0.25明显高于未临产组1.94±0.23,差异有统计学意义(P0.05)。临产组血清E2、E3浓度[(7882.41±921.76)pmol/L,(544.12±15.58)nmol/L]均显著高于未临产组[(5210.72±873.13)pmol/L,(326.36±12.26)nmol/L]。结论足月妊娠分娩发动时GPR30高表达可能参与介导雌激素对子宫收缩的调节作用。
[Abstract]:Objective to investigate the expression of G protein coupled receptor 30(GPR30)m RNA and protein in uterine smooth muscle tissue before and after labor and their correlation with labor initiation. Methods from March 2011 to March 2012, full-term pregnant women who underwent selective cesarean section before labor were selected as non-delivery group in Qingdao City Hospital. The pregnant women undergoing emergency cesarean section were given emergency cesarean section after entering the active stage of labor. A pregnant woman, as a parturient group, took a small amount of uterine smooth muscle tissue during cesarean section. The expression of GPR30 m RNA and protein in uterine smooth muscle tissue was detected by real-time fluorescence quantitative PCR and Western blot, and the serum levels of estradiol E2P, estriol E3 and progesterone were measured by radioimmunoassay. Results the expression of GPR30 m RNA was detected by real-time fluorescence quantitative PCR in uterine smooth muscle tissues of parturient and non-parturient groups. Ct was 3.08 卤0.35 in parturient group and 4.02 卤0.67 in non-delivery group. The difference was statistically significant (P 0.05). The expression of GPR30 m RNA in parturient group was 1.92 times higher than that in non-parturient group. The expression of GPR30 protein was detected by Western blot in uterine smooth muscle tissue of parturient group and non-parturient group. The relative expression of GPR30 protein in parturient group was 4.06 卤0.25 significantly higher than that in non-parturient group (1.94 卤0.23), and the difference was statistically significant (P 0.05). The serum E2N E3 concentration in parturient group [7882.41 卤921.76pmol / L, 544.12 卤15.58)nmol/L] was significantly higher than that in non-delivery group [5210.72 卤873.13pmol / L, 326.36 卤12.26)nmol/L]. Conclusion the high expression of GPR30 during term pregnancy may be involved in the regulation of uterine contraction by estrogen.
【作者单位】：青岛市市立医院产科;
【分类号】：R714

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