武汉市某医院筛查女性人乳头瘤病毒感染及基因型分布特点分析

发布时间：2018-04-22 16:43

  本文选题：人乳头瘤病毒 + 基因型　； 参考：《中华肿瘤防治杂志》2015年19期

【摘要】：目的分析武汉市第五医院筛查的女性生殖道人乳头瘤病毒(human papillomavirus,HPV)的感染状况以及其分布特点,为女性宫颈癌的防治及HPV疫苗研制提供流行病学依据。方法收集2011-05-01-2014-09-30武汉市第五医院体检或妇科就诊的女性宫颈脱落细胞样本共2 409份,采用导流杂交基因芯片技术对样本进行HPV基因分型检测,检测HPV16、18、31、33、35、39、45、51、52、53、56、58、59、66和68共15种高危亚型和HPV6、11、42、43、44和81共6种低危亚型。按年龄将研究对象分为5组,18~30岁、30~40岁、40~50岁、50~60岁和60岁组,并对HPV感染病例的年龄分布以及基因型进行分析。结果共检测样本2 409例,检出HPV阳性样本422例,HPV阳性检出率为17.51%。21种HPV亚型中共检出20种亚型,低危型HPV43未检出,其中HPV高危亚型约占82.76%。HPV高危亚型按检出频次由高到低依次为16(19.24%)、52(15.63%)、58(10.42%)、53(6.61%)、33(5.61%)、39(5.01%)、18(4.81%)、31(4.01%)、68(3.41%)、66(2.61%)、59(2.20%)、56(1.40%)、51(1.00%)、35(0.60%)和45(0.20%),HPV低危亚型由高到低依次为81(8.22%)、11(5.21%)、6(2.40%)、44(1.00%)和42(0.40%)。感染类型以单一亚型感染为主,约占83.64%,其中高危亚型检出率达到83.56%。单一亚型感染病例中的高危亚型检出率低于其他2种感染类型,P0.05。50~60岁人群的阳性检出率(22.57%)高于其他年龄组的阳性检出率,P0.05。40~50岁人群HPV感染优势亚型首位为HPV52,不同于其余各年龄段人群HPV感染的首位优势亚型(HPV16)。结论武汉市第五医院筛查的女性生殖道HPV感染的主要亚型为高危亚型HPV16、52。感染类型以单一亚型感染为主,其中高危亚型检出率最高。50~60岁人群的HPV阳性检出率高于其他年龄分组,临床应重视该年龄段人群的HPV筛查。
[Abstract]:Objective to analyze the status and distribution of human papillomavirus (HPV) infection in female genital tract human papillomavirus (HPV) screened in the Fifth Hospital of Wuhan, and to provide epidemiological evidence for the prevention and treatment of female cervical cancer and the development of HPV vaccine. Methods A total of 2 409 samples of female cervical exfoliated cells were collected from the fifth hospital of Wuhan from 2011-05-01-2014-09-30. The HPV genotyping was detected by using diversion hybridization gene chip technique. A total of 15 high risk subtypes of HPV16, HPV6112A42O43M44 and 81 were detected for HPV16 (18 / 31), 3' 3' / 3' / 3' / 451 / 551, 51n / 52N, 52N, 58N / 596, 68, and HPV6111142A43M44 and 81, respectively, in which 6 low risk subtypes were detected. The subjects were divided into 5 groups according to age. The age distribution and genotype of HPV infection cases were analyzed. Results A total of 2 409 cases were detected. The positive rate of HPV in 422 cases was 17.51%. 20 subtypes were detected in 21 HPV subtypes, but 20 subtypes were not detected in low-risk HPV43. 鍏朵腑HPV楂樺嵄浜氬瀷绾﹀崰82.76%.HPV楂樺嵄浜氬瀷鎸夋��鍑洪�戞�＄敱楂樺埌浣庝緷娆′负16(19.24%),52(15.63%),58(10.42%),53(6.61%),33(5.61%),39(5.01%),18(4.81%),31(4.01%),68(3.41%),66(2.61%),59(2.20%),56(1.40%),51(1.00%),35(0.60%)鍜，

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