抑制VEGF-B表达对人绒毛膜癌裸鼠移植瘤影响的研究

发布时间：2018-04-22 17:06

本文选题：RNA干扰 + VEGF-B　；参考：《现代妇产科进展》2015年02期

【摘要】：目的:研究RNA干扰下调血管内皮生长因子-B(VEGF-B)表达对人绒毛膜癌裸鼠移植瘤生长、侵袭转移及血管生成的影响。方法:将构建的含VEGF-B shRNA的质粒转染人绒毛膜癌细胞JEG-3,建立裸鼠皮下移植瘤模型。观察shRNA干扰VEGF-B表达对JEG-3裸鼠移植瘤生长及侵袭转移能力的影响,并采用免疫组化技术检测移植瘤组织中VEGF-B的表达变化及其所含CD34标记的肿瘤微血管密度(MVD)的差异。结果:(1)抑制VEGF-B表达可减小裸鼠皮下肿瘤大小(P0.05),并降低肿瘤细胞肺转移的发生率(P0.05);(2)免疫组化结果提示,VEGF-B shRNA转染组移植瘤中VEGF-B表达及MVD均低于空白对照组和阴性对照组(P0.05);(3)相关性分析显示,VEGF-B蛋白表达水平与绒毛膜癌MVD高低成正相关。结论:VEGF-B基因在绒毛膜癌的体内生长、侵袭转移中起重要作用,shRNA下调其表达能抑制绒毛膜癌细胞的体内生长、侵袭转移,可能与其调控瘤组织内的微血管形成有关。
[Abstract]:Aim: to investigate the effect of RNA interference on the growth, invasion and metastasis and angiogenesis of human choriocarcinoma xenografts in nude mice. Methods: the constructed plasmid containing VEGF-B shRNA was transfected into human choriocarcinoma cell line JEG-3 to establish subcutaneous transplanted tumor model in nude mice. To observe the effect of shRNA interference VEGF-B expression on the growth, invasion and metastasis of JEG-3 xenografts in nude mice, and to detect the changes of VEGF-B expression in transplanted tumor tissues and the difference of microvessel density (MVD) labeled with CD34 by immunohistochemical technique. Results 1) inhibition of VEGF-B expression could reduce the size of subcutaneous tumor in nude mice and decrease the incidence of lung metastasis in nude mice. The immunohistochemical results showed that the expression of VEGF-B and MVD in transplanted tumor in VEGF-B shRNA transfection group were lower than those in blank control group and negative control group. The correlation analysis showed that the expression of VEGF-B protein was positively correlated with the level of MVD in choriocarcinoma. Conclusion VEGF-B gene plays an important role in the growth and metastasis of choriocarcinoma. Down-regulation of shRNA expression can inhibit the growth of choriocarcinoma cells in vivo, invasion and metastasis, which may be related to the regulation of microangiogenesis in tumor tissue.
【作者单位】：中南大学湘雅医院妇产科;湖南省肿瘤医院;中南大学湘雅医学院附属肿瘤医院;
【基金】：国家自然科学基金项目资助(No:81472434)
【分类号】：R737.33

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