Forskolin对人羊膜WISH细胞中AQP8、AQP9的干预作用

发布时间：2018-04-22 20:46

本文选题：水通道蛋白8 + 水通道蛋白9　；参考：《遵义医学院》2014年硕士论文

【摘要】：目的：观察腺苷酸环化酶激动剂Forskolin对人羊膜上皮细胞株（WISH细胞）中水通道蛋白8（AQP8）与水通道蛋白9（AQP9）的干预作用。方法：对人羊膜上皮细胞株（WISH细胞）进行体外培养，将其随机分为不同浓度Forskolin组（0μmol/L即对照组，1μmol/L，10μmol/L，，50μmol/L，100μmol/L，200μmol/L），作用24小时，运用实时荧光定量（real time RT-PCR）及蛋白印迹（western blot）技术检测AQP8、AQP9的mRNA及蛋白表达水平。结果：不同浓度Forskolin作用组结果显示，1μmol/L与对照组（0μmol/L）比较，P0.05,无明显差异，即1μmol/L Forskolin对AQP8、9的作用不明显，其余各浓度组与对照组比较，P0.05，有显著差异，且随浓度梯度呈上升趋势，在100μmol/L时达到高峰，200μmol/L时下降，但仍高于对照组。结论：腺苷酸环化酶激动剂Forskolin对人羊膜WISH细胞中AQP8、AQP9的mRNA及蛋白具有上调作用，且具有一定的剂量依赖性。
[Abstract]:Objective: To observe the effect of adenylate cyclase agonist Forskolin on water channel protein 8 (AQP8) and aquaporin 9 (AQP9) in human amniotic epithelial cell line (WISH cells).
Methods: the human amniotic epithelial cell line (WISH cell) was cultured in vitro. It was randomly divided into groups of different concentration Forskolin (0 mu mol/L, 1 mol/L, 10 mu mol/L, 50 mu mol/L, 100 mu mol/L, 200 mu mol/L), and used real time fluorescence quantitative (real time RT-PCR) and Western blotting. And protein expression level.
Results: the results of different concentrations of Forskolin group showed that 1 mu mol/L was compared with the control group (0 mu mol/L), and there was no obvious difference in P0.05. That is, the effect of 1 mu mol/L Forskolin on AQP8,9 was not obvious. The other concentration groups were compared with the control group, and there were significant differences between the other groups and the control group, and with the increase of the concentration ladder, the peak reached the peak at 100 u mol/L and 200 mu mol/L. But it was still higher than the control group.
Conclusion: adenylate cyclase agonist Forskolin has a dose-dependent effect on the AQP8 and AQP9 mRNA and protein in human amniotic WISH cells.

【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.7

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