MicroRNA-138与microRNA-720在新疆维吾尔族HPV16相关宫颈鳞癌中表达的研究

发布时间：2018-04-23 05:29

  本文选题：维吾尔族 + 宫颈鳞癌　； 参考：《新疆医科大学》2014年硕士论文

【摘要】：目的：探讨microRNA-138、microRNA-720在新疆南部维吾尔族HPV16相关宫颈鳞癌组织中表达的差异。方法：采用琼脂糖凝胶电泳技术检测4例宫颈癌细胞系、83例新疆南部维吾尔族宫颈鳞癌组织中HPV16的表达,采用实时定量RT-PCR技术对4例宫颈癌细胞系、83例新疆南部维吾尔族宫颈鳞癌组织标本、16例与之对应的癌旁组织标本及40例新疆南部维吾尔族正常宫颈组织标本检测目的基因microRNA-138、microRNA-720的表达。结果：与癌旁组相比,microRNA-138、 microRNA-720在新疆南部维吾尔族宫颈鳞癌组中的表达均下调,差异有统计学意义(P0.05)；与正常宫颈组相比,microRNA-138、microRNA-720在新疆南部维吾尔族宫颈鳞癌组中的表达均下调,差异有统计学意义(P0.05)；与HPV16阴性宫颈鳞癌组相比,microRNA-138、microRNA-720在HPV16阳性新疆南部维吾尔族宫颈鳞癌组中的表达均有下调趋势,但差异无统计学意义(P0.05)；与HPV16阴性宫颈癌细胞系相比,microRNA-138、microRNA-720在HPV16阳性宫颈癌细胞系中的表达无明显差异(P0.05)。而且,下调的miRNA-138、miRNA-720均促进淋巴结转移、脉管浸润(P0.05),但与年龄、宫颈壁累及范围无明显关系(P0.05)；miRNA-720与临床分期、肿瘤体积有关(P0.05)；但miRNA-138与临床分期、肿瘤体积无明显相关性(P0.05)。结论：MiRNA-138、miRNA-720与新疆南部维吾尔族宫颈鳞癌密切相关,并与淋巴结转移、脉管浸润有关,但与HPV16无明显相关性。miRNA-720与临床分期、肿瘤体积也具有相关性。
[Abstract]:Objective: to investigate the expression of microRNA-138C microRNA-720 in Uygur cervix squamous cell carcinoma (SCC) tissues of Uygur nationality in southern Xinjiang. Methods: the expression of HPV16 was detected by agarose gel electrophoresis in 83 cases of cervical squamous cell carcinoma of Uygur nationality in southern Xinjiang. Real-time quantitative RT-PCR technique was used to detect 4 cervical cancer cell lines and 83 specimens of cervical squamous cell carcinoma tissues from Uygur nationality in southern Xinjiang. 16 specimens from adjacent tissues and 40 specimens from normal tissues of Uygur nationality in southern Xinjiang were detected. Objective to express the microRNA-720 gene. Results: compared with the paracancerous group, the expression of microRNA-720 was down-regulated in Uygur cervix squamous cell carcinoma group in the south of Xinjiang, the difference was statistically significant (P 0.05), and the expression of microRNA-138 microRNA-720 was down-regulated in Uygur cervix squamous cell carcinoma group in the south of Xinjiang compared with the normal cervix group. The expression of microRNA-138 microRNA-720 was down-regulated in HPV16 positive Uygur cervix cervix squamous cell carcinoma group in southern Xinjiang. However, there was no significant difference in the expression of microRNA-138 microRNA-720 in HPV16 positive cervical cancer cell line compared with HPV16 negative cervical cancer cell line. Moreover, the down-regulated miRNA-138miRNA-720 promoted lymph node metastasis and vessel invasion (P0.05), but had no significant relationship with age, cervical wall involvement and clinical stage, tumor volume was related to P0.05, but miRNA-138 had no significant correlation with clinical stage. ConclusionMiRNA-138miRNA-720 is closely related to squamous cell carcinoma of cervix of Uygur cervix in southern Xinjiang, and is related to lymph node metastasis and vascular invasion, but not to HPV16. MiRNA-720 is correlated with clinical stage and tumor volume.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

【参考文献】

相关期刊论文 前10条

1 陈军莹;姚德生;;microRNA在宫颈癌中的研究进展[J];重庆医学;2011年15期

2 胡淼;欧阳玲;;microRNA在女性生殖系统常见疾病中的研究进展[J];国际生殖健康/计划生育杂志;2012年01期

3 陈锐;李庭芳;王新玲;李莉;程静新;;新疆自治区宫颈浸润癌2417例临床流行病学回顾性分析[J];中华肿瘤防治杂志;2008年05期

4 刘浩;尹华斌;纪方;;MicroRNA与肿瘤的相关研究进展[J];现代生物医学进展;2012年07期

5 徐晓慧;陈照丽;赫捷;;microRNA-106b-25在恶性肿瘤发生中的作用[J];现代肿瘤医学;2010年12期

6 林淼;薛寅佳;戎瑞明;朱同玉;;小RNA(miRNA)在辅助性T(Th)细胞发育及功能中的重要作用[J];复旦学报(医学版);2012年03期

7 曹长运;孙凤林;;MicroRNA和肿瘤[J];中国医药导报;2012年14期

8 姜淑清;王涛;土送爱;周俊兰;买热木沙汗;徐雪;海日尼沙汗;茹仙姑丽;沙来买提;艾木热汗;邓小虹;;新疆策勒县宫颈癌的流行病学调查研究[J];中国实用妇科与产科杂志;2006年05期

9 陈寿仁;王占祥;陈玉英;;microRNA与胶质瘤侵袭性关系的研究进展[J];肿瘤防治研究;2011年01期

10 吴留洋;霍钢;杜安东;肖文峰;张铃铛;;miRNA在侵袭性垂体腺瘤中表达的初步研究[J];中国神经精神疾病杂志;2011年12期

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