组织因子途径抑制物-2在宫颈癌中的表达及其对宫颈癌细胞增殖、凋亡的影响

发布时间：2018-04-25 00:09

本文选题：宫颈癌 + 组织因子途径抑制物-　；参考：《中国老年学杂志》2015年01期

【摘要】：目的观察组织因子途径抑制物(TFPI)-2在正常宫颈、宫颈上皮内肿瘤(CIN)和宫颈鳞癌组织中表达的差异及TFPI-2对体外培养的宫颈癌Hela细胞增殖和凋亡的影响。方法收集2009~2010年在该院妇产科住院治疗的68例宫颈鳞癌患者,48例CIN患者及12例宫颈正常患者的宫颈组织标本,免疫组化染色检测宫颈组织中TFPI-2的表达。取生长状态良好的宫颈癌Hela细胞,应用基因转染的方法建立TFPI-2高表达细胞系,Real-time PCR和Western印迹方法检测基因转染前后细胞中TFPI-2 mRNA和蛋白的表达;四甲基偶氮唑蓝比色法(MTT)检测各组细胞的增殖活性,流式细胞仪检测细胞凋亡。结果 1免疫组化染色显示,正常宫颈组织、CIN和宫颈癌中TFPI-2表达逐渐降低,三者间比较差异有统计学意义(P0.01)。2体外观察发现,转染pc DNA3.1-TFPI-2的Hela-TFPI-2组细胞TFPI-2 mRNA和蛋白表达明显高于转染空白质粒组和空白对照组(P0.01);MTT结果显示,Hela-TFPI-2组细胞增殖速度明显低于空白对照组和空白质粒组,且随着培养时间的延长,这种抑制作用更明显(P0.05);流式细胞仪检测结果显示Hela-TFPI-2组细胞凋亡率显著高于空白对照组和空白质粒组(P0.05)。结论 TFPI-2表达随着宫颈病变的进展逐渐降低;同时过表达的TFPI-2可明显抑制宫颈癌Hela细胞增殖,促进其凋亡。提示TFPI-2的表达减少可能是宫颈癌发生发展的机制之一,TFPI-2可能是治疗宫颈癌的新靶点。
[Abstract]:Objective to investigate the expression of tissue factor pathway inhibitor (TFPI-2) in normal cervix, cervical intraepithelial neoplasms (cin) and cervical squamous cell carcinoma (SCC) and the effect of TFPI-2 on proliferation and apoptosis of Hela cells cultured in vitro. Methods from 2009 to 2010, 68 cases of cervical squamous cell carcinoma (CIN) and 12 cases of normal cervix were collected from our hospital. The expression of TFPI-2 in cervical tissues was detected by immunohistochemical staining. The expression of TFPI-2 mRNA and protein in cervical cancer Hela cells with good growth state was detected by real-time PCR and Western blotting. The proliferation activity and apoptosis of the cells in each group were detected by MTT assay and flow cytometry respectively. Results 1Immunohistochemical staining showed that the expression of TFPI-2 in normal cervical tissue and cervical carcinoma decreased gradually, and the difference between them was statistically significant. The expression of TFPI-2 mRNA and protein in the Hela-TFPI-2 group transfected with PC DNA3.1-TFPI-2 was significantly higher than that in the blank plasmid group and the blank control group. The results showed that the proliferation rate of the cells in the Hela-TFPI-2 group was significantly lower than that in the blank control group and the blank plasmid group, and with the extension of culture time, the proliferation rate of the cells in the control group was significantly lower than that in the control group. The results of flow cytometry showed that the apoptosis rate of Hela-TFPI-2 group was significantly higher than that of blank control group and blank plasmid group. Conclusion the expression of TFPI-2 decreased gradually with the progression of cervical lesions, and overexpression of TFPI-2 could significantly inhibit the proliferation and promote the apoptosis of cervical cancer Hela cells. The results suggest that the decrease of TFPI-2 expression may be one of the mechanisms of cervical carcinogenesis and development. TFPI-2 may be a new target for the treatment of cervical cancer.
【作者单位】：沈阳医学院附属中心医院妇产科;
【分类号】：R737.33

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