妊娠期及哺乳期的持续性低蛋白营养不良易增加宫内发育迟缓大鼠糖代谢异常风险

发布时间：2018-04-25 21:30

本文选题：宫内发育迟缓 + 追赶生长　；参考：《实用医学杂志》2015年16期

【摘要】：目的:评估不同生长发育时期予不同饮食干预下宫内发育迟缓(IUGR)大鼠糖代谢状态及血脂水平。方法:妊娠SD母鼠随机予正常饮食(normal diet,ND)(A组)及低蛋白饮食(low protein diet,LPD)(B组)。分娩后,A组雄性新生子鼠哺乳期(during lactation,DL)其母鼠予ND,断乳后(after lactation,AL)子鼠仍予ND(Con组);B组雄性子鼠筛选出IUGR鼠。IUGR鼠予不同饮食干预:(1)DL母鼠仍予LPD,AL子鼠予高脂饮食(high fat diet,HFD)(IUGR+L+H组);(2)DL母鼠予HFD,AL子鼠予HFD(IUGR+H+H组);(3)DL母鼠予ND,AL子鼠予ND(IUGR+N组)。采用口服葡萄糖耐量试验(OGTT)及胰岛素耐量试验(ITT)评估糖代谢状况及检测血脂水平。结果:Lee′s指数示各组IUGR雄鼠均大于Con组(均P0.05)。OGTT显示糖负荷后30 min,IUGR+L+H组血糖高于Con组(P0.05);120 min时,IUGR+L+H组血糖高于Con组(P0.05)。IUGR+L+H组AUCglucose高于Con组(P0.05)。ITT显示Insulin负荷后30 min,IUGR+L+H组血糖下降速率百分比低于Con组(P0.05)。IUGR+L+H组HDL低于Con组(P0.05)。结论 :IUGR雄鼠不同饮食干预成年后易出现体重超重甚至肥胖。母鼠妊娠期及哺乳期持续性低蛋白营养不良易增加子鼠糖脂代谢紊乱风险。
[Abstract]:Aim: to evaluate the glucose metabolism and blood lipid levels in IUGRs of intrauterine growth retardation (IUGR) rats treated with different dietary interventions during different growth and development periods. Methods: pregnant SD rats were randomly assigned to normal diet (group A) and low protein diet (group B). After delivery male newborn mice in group A were given NDD during lactation and after weaning after lactationDLL) the offspring of group A were still given ND(Con group after lactation.Infant group B, IUGR mice were screened out with different dietary interventions. The female mice were given high fat diettid HFDL H, which were still given to LPDAL mice with different dietary interventions. The female rats in group A were treated with HFD(IUGR H H group (HFD(IUGR H group) and ND(IUGR N group (P < 0.05). Oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) were used to assess glucose metabolism and blood lipid level. Results the IUGR male rats in each group were higher than those in the Con group (all P0.05).OGTT showed that the blood glucose in the P0.05).OGTT group was higher than that in the Con group at 30 min after glucose load (P0.05). The level of blood glucose in the IUGR-H group was higher than that in the Con group (P 0.05). The AUCglucose in the IUGR-L group was higher than that in the Con group (P 0.05 .ITT). 30 min after Insulin load, the level of blood glucose in the IUGR-H group was higher than that in the Con group. The percentage of decreasing rate of blood glucose in group H was lower than that in group Con (P 0.05). The percentage of HDL in group H was lower than that in group Con (P 0.05). Conclusion the male rats with different diets may be overweight or obese after adulthood. Persistent low-protein malnutrition during pregnancy and lactation increased the risk of glucose and lipid disorders in the offspring.
【作者单位】：汕头大学医学院第一附属医院内分泌科;
【基金】：国家自然科学基金(编号:81170711)
【分类号】：R714.2

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