人脐带间充质干细胞移植治疗小鼠复发性自然流产效果及其机制探讨

发布时间：2018-04-26 22:25

本文选题：人脐带间充质干细胞 + 自然流产　；参考：《山东医药》2017年03期

【摘要】：目的观察人脐带华通氏胶间充质干细胞(WJ-MSCs)移植治疗复发性自然流产小鼠的效果,并探讨其机制。方法雌性CBA/J小鼠与雄性BALB/c小鼠按1∶1比例合笼交配,制作正常妊娠小鼠模型(对照组,5只)。雌性CBA/J小鼠与雄性DBA/2小鼠按1∶1比例合笼交配,制作复发性自然流产小鼠模型20只,分为模型组、实验1组、实验2组、实验3组,每组各5只。模型组于孕1天经尾静脉注射DMEM/F12培养基0.2 mL;各实验组于孕第1天经尾静脉注射WJ-MSCs 0.2 mL(6×107个/mL),其中实验1组仅注射1次,实验2组隔天注射1次、连续2次,实验3组隔天注射1次、连续3次。分别于孕第14天处死各组小鼠,计算胚胎吸收率;取小鼠蜕膜及胎盘组织,采用Western blotting法检测IL-10、IL-17、IFN-γ蛋白,qRT-PCR法检测IL-10、IL-17、IFN-γmRNA。结果模型组及实验1组胚胎吸收率高于对照组,实验3组胚胎吸收率均低于模型组和实验1组(P均0.05)。实验1、2组IL-17蛋白相对表达量高于对照组,实验3组低于对照组(P均0.05)。实验1组蜕膜及胎盘组织中IFN-γmRNA相对表达量高于对照组,实验2、3组低于对照组(P均0.05)。实验1、2、3组蜕膜及胎盘组织中IL-10 mRNA和蛋白相对表达量高于模型组,IL-17、IFN-γmRNA和蛋白相对表达量低于模型组(P均0.05)。实验1、2、3组蜕膜及胎盘组织中的IL-10 mRNA和蛋白相对表达量递增,IL-17 mRNA和蛋白、IFN-γ蛋白相对表达量递减,除实验2、3组IFN-γmRNA表达差异无统计学意义外,各组其余指标相比,P均0.05。结论 WJ-MSCs移植后复发性自然流产模型小鼠的胚胎吸收率降低,其机制可能与上调母-胎界面IL-10表达、下调IL-17及IFN-γ表达有关。
[Abstract]:Objective to observe the effect of WJ-MSCs transplantation on recurrent spontaneous abortion in mice with human umbilical cord Huatong's gum mesenchymal stem cells (WJ-MSCs) and to explore its mechanism. Methods female CBA/J mice and male BALB/c mice were mated in a cage at 1:1 to make normal pregnant mice model (control group, 5 mice). A total of 20 female CBA/J mice and male DBA/2 mice were mated in a cage at 1:1. The mice were divided into three groups: model group, experimental group 1, experimental group 2 and experimental group 3, each with 5 mice in each group. On the first day of gestation, the model group was injected with DMEM/F12 medium (0.2 mL) via caudal vein, and each experimental group was injected with WJ-MSCs 0.2 mL(6 脳 107 mLL on the first day of pregnancy. The experimental group 1 was injected only once, and the second group was injected once every other day for 2 consecutive times. Group 3 was injected once every other day for 3 consecutive times. On the 14th day of gestation, the mice were killed to calculate the embryo absorption rate, and the decidua and placenta of the mice were collected, and IL-10 IL-17 IFN- 纬 protein was detected by Western blotting method, and IL-10 IL-17 IFN- 纬 mRNA was detected by qRT-PCR. Results the embryo absorptivity of model group and experimental group 1 was higher than that of control group, and the embryo absorption rate of experimental group 3 was lower than that of model group and experiment 1 group (P < 0.05). In experiment 1, the relative expression of IL-17 protein in group 2 was higher than that in control group, and that in group 3 was lower than that in control group (P < 0.05). The relative expression of IFN- 纬 mRNA in decidua and placenta of experimental group 1 was higher than that of control group, and the level of IFN- 纬 mRNA in group 2 was lower than that in control group (P 0.05). In experiment 1, the relative expression of IL-10 mRNA and protein in decidua and placenta was higher than that in model group. The relative expression of IFN- 纬 mRNA and protein in decidua and placenta was lower than that in model group (P < 0.05). In experiment 1, the relative expression of IL-10 mRNA and protein in decidua and placenta was increased and the relative expression of IL-17 mRNA and protein IFN- 纬 was decreased. Except for experiment 2, there was no significant difference in the expression of IFN- 纬 mRNA in the three groups, and the other indexes in each group were 0.05 compared with each other (P < 0.05). Conclusion the embryo absorption rate of recurrent spontaneous abortion model mice after WJ-MSCs transplantation is decreased, which may be related to the up-regulation of IL-10 expression and down-regulation of IL-17 and IFN- 纬 expression at the maternal and fetal interface.
【作者单位】：南通大学附属医院;
【基金】：国家自然科学基金青年基金资助项目(81401272)
【分类号】：R714.21

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