老年宫颈癌组织中人乳头状瘤病毒16-E6、p53 mRNA表达及其与放疗敏感性的相关性

发布时间：2018-04-28 02:50

本文选题：宫颈癌 + 放疗敏感性　；参考：《中国老年学杂志》2017年22期

【摘要】：目的分析老年宫颈癌组织中人乳头状瘤病毒(HPV)16-E6、p53 mRNA表达及其与放疗敏感性的关系。方法 40例宫颈癌患者均按计划进行根治性放射治疗前后采用实时定量反转录聚合酶链反应(PT-PCR)检测宫颈癌组织中HPV16-E6、p53 mRNA的表达水平,并根据治疗结果分为有效组29例和无效组11例,分析HPV16-E6、p53与放疗敏感性的关系。结果治疗总有效率为72.50%;放疗前后无效组2例HPV16-E6、p53 mRNA和蛋白差异无统计学意义(P0.05),放疗前后有效组4例HPV16-E6 mRNA、p53 mRNA表达水平明显改善,HPV16-E6蛋白表达水平显著低于无效组,p53蛋白表达水平显著高于无效组(P0.05)。结论 HPV16-E6感染、p53基因异常参与了老年宫颈癌发生、发展过程,检测HPV16-E6、p53 mRNA的表达可判断宫颈癌组织放疗敏感性。
[Abstract]:Objective to analyze the expression of human papillomavirus (HPV) 16-E6 mRNA and its relationship with radiosensitivity in elderly patients with cervical cancer. Methods the expression of HPV16-E6 p53 mRNA was detected by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) before and after radical radiotherapy in 40 patients with cervical cancer. According to the results of treatment, we divided into effective group (29 cases) and ineffective group (11 cases). The relationship between HPV16-E6 p53 and radiosensitivity was analyzed. Results the total effective rate was 72.50%, there was no significant difference in mRNA and protein of HPV16-E6 p53 in 2 cases of ineffective group before and after radiotherapy, while the expression level of HPV16-E6 mRNA-p53 mRNA in 4 cases of effective group before and after radiotherapy was significantly lower than that of non-effective group (P 0.05). The expression level was significantly higher than that in ineffective group (P 0.05). Conclusion the abnormal expression of p53 gene in HPV16-E6 infection may play an important role in the occurrence and development of cervical carcinoma in the elderly. Detection of p53 mRNA expression in HPV16-E6 gene may be used to judge the sensitivity of cervical carcinoma to radiotherapy.
【作者单位】：山东大学附属济南市中心医院;
【分类号】：R737.33

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