高效液相色谱法同时测定妊娠合并乙型肝炎患者血浆中的吲哚与3-甲基吲哚

发布时间：2018-04-29 07:53

本文选题：高效液相色谱法 + 吲哚　；参考：《色谱》2017年07期

【摘要】：建立了一种高效液相色谱-荧光检测法用于同时测定血浆中的吲哚与3-甲基吲哚。样本经液液萃取法提取,采用Shim-Pack VP-ODS柱(150 mm×4.6 mm,4.6μm),以15 mmol/L磷酸二氢钠溶液-甲醇(40∶60,v/v)为流动相,甲奈酚为内标,荧光激发和发射波长分别为274 nm和340 nm。吲哚和3-甲基吲哚的线性范围分别为2.22~88.89μg/L和1.11~44.44μg/L;检出限分别为0.11μg/L(吲哚)和0.06μg/L(3-甲基吲哚);平均回收率为95.5%~112.3%,日内与日间相对标准偏差均小于6.8%。利用该方法对妊娠合并乙肝患者(n=29)和正常孕妇(n=46)的血浆进行了测定,结果表明妊娠合并乙肝患者血浆中吲哚和3-甲基吲哚水平均显著高于正常对照组,且与肝损伤指标转氨酶水平呈正相关。
[Abstract]:A high performance liquid chromatography-fluorescence detection method was developed for simultaneous determination of indole and 3-methylindole in plasma. The samples were extracted by liquid-liquid extraction, and the Shim-Pack VP-ODS column was used to extract the samples. The mobile phase was 15 mmol/L sodium dihydrogen phosphate solution-methanol 40: 60 v / v). The fluorescence excitation and emission wavelengths were 274nm and 340nm, respectively. The linear ranges of indole and 3-methylindole were 2.22 ~ 88.89 渭 g / L and 1.1144.44 渭 g / L, respectively, the detection limits were 0.11 渭 g / L (indole) and 0.06 渭 g / L ~ (3-methylindole), the average recovery was 95.5 渭 g / L ~ (-1) and the relative standard deviation between day and day was less than 6.8 渭 g 路L ~ (-1). The plasma levels of indoles and 3-methylindole in pregnant women with hepatitis B were significantly higher than those in normal controls. There was a positive correlation between the level of transaminase and liver injury index.
【作者单位】：重庆医科大学检验医学院临床检验诊断学教育部重点实验室;重庆医科大学附属第一医院内分泌科;重庆医科大学公共卫生与管理学院;重庆医科大学附属第一医院检验科;
【基金】：国家自然科学基金青年基金(81200294)~~
【分类号】：O657.72;R714.251

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